基于线粒体COⅠ基因和控制区序列的辽宁沿海弯棘斜棘鳂群体遗传多样性和遗传结构分析  

POPULATION GENETIC STRUCTURE AND DIVERSITY ANALYSIS OF REPOMUCENUS CURVICORNIS IN THE LIAONING COAST BASED ON DNA SEQUENCES OF THE MITOCHONDRIAL COⅠ GENE AND CONTROL REGION

作　　者：李玉龙[1] 刘修泽[1] 于旭光[1] 李轶平[1] 付杰[1] 董婧[1] 

机构地区：[1]辽宁省海洋水产科学研究院,辽宁省海洋生物资源与生态学重点实验室,大连116023

出　　处：《水生生物学报》2017年第3期581-588,共8页Acta Hydrobiologica Sinica

基　　金：海洋公益性行业科研专项(201405010);辽宁省海洋与渔业科研项目(201401)资助~~

摘　　要：为研究辽宁沿海弯棘斜棘逐(Repomucenus curvicornis)自然群体的遗传多样性及遗传结构,采用PCR扩增获得辽宁沿海弯棘斜棘逐辽东湾群体(n=22)及黄海北部群体(n=18)线粒体的COⅠ及控制区(CR)部分DNA序列片段,进行序列比较及遗传多样性分析。获得弯棘斜棘逐COⅠ基因片段624 bp,其A、T、C、G平均含量分别为24.09%、31.04%、25.28%和19.59%;CR片段460 bp,其A、T、C、G平均含量分别为32.96%、32.80%、14.86%和19.38%。基于COⅠ基因和CR序列得到的两群体变异位点数、平均核苷酸差异数、单倍型多样性指数以及核苷酸多样性指数分别为:38、4.67、0.96±0.02和0.0075±0.0042;26、3.35、0.97±0.02和0.0073±0.0043。序列分析结果均显示,辽东湾群体的遗传多样性低于黄海北部群体。分子方差(AMOVA)分析结果显示,基于COⅠ基因片段辽东湾与黄海北部群体间无明显遗传分化(F_(st)=0.0091,P=0.25)而基于CR序列两群体间具有较小但接近显著的遗传分化(F_(st)=0.0264,P=0.09)。研究表明,线粒体CR序列与COⅠ基因均可作为检测弯棘斜棘逐群体遗传多样性的有效分子标记,但CR序列遗传分化的敏感度要高于COⅠ基因,更适合作为弯棘斜棘逐群体遗传研究的分子标记。To analyze the genetic diversity and genetic structure of Repomucenus curvicornis from the Liaodong Bay（n=22） and the North Yellow Sea （n=18）, the mitochondrial DNA cytochrome oxidase I （COⅠ） gene and control region fragments were obtained by PCR amplification. The average contents of A, T, C and G of 624 bp COⅠgene fragments were 24.09%, 31.04%, 25.28%, and 19.59%, respectively. The average contents of A, T, C and G of CR fragments （460 bp） were 32.96%, 32.80%, 14.86% and 19.38%, respectively. The total variable sites, mean pairwise nucle-otide differences （k）, haplotype diversity （H） and nucleotide diversity （π） based on COⅠgene fragments were 38, 4.67,0.96±0.02 and 0.0075±0.0042, respectively. The same parameters based on CR fragments were 26, 3.35, 0.97±0.02 and0.0073±0.0043, respectively. Based on mitochondrial COⅠgene and control region, the genetic diversity of LiaodongBay population was lower than that of North Yellow Sea population. The AMOVA analysis based on CR fragments revealed almost significant genetic divergence between the Liaodong Bay and North Yellow Sea populations, while therewas no significant genetic divergence based on COⅠgene. The results showed that CR and COⅠgene were effectivemolecular markers for detecting the genetic diversity of Repomucenus curvicornis population, while CR was more reliable than COⅠgene in detecting the genetic structure. In conclusion, CR is a appropriate marker for marine fish population genetic analysis.

关 键 词：弯棘斜棘逐 线粒体DNA COⅠ基因 控制区序列 遗传多样性 遗传分化 

分 类 号：Q346.5[生物学—遗传学]