DNA条形码在淡水红藻中的应用评价——基于串珠藻科植物（英文）  

EVALUATING DNA BARCODE MARKERS FOR FRESHWATER RED ALGAE: A CASE STUDY USING FAMILY BATRACHOSPERMACEAE

作　　者：吉莉[1] 冯佳[2] 南方茹 陈乐[2] 胡变芳[3] 谢树莲[2] 

机构地区：[1]太原科技大学环境与安全学院,太原030024 [2]山西大学生命科学学院,太原030006 [3]晋中学院生物科学与技术学院,晋中030600

出　　处：《水生生物学报》2017年第3期643-651,共9页Acta Hydrobiologica Sinica

基　　金：Supported by the National Natural Science Foundation of China(31370239,31440026);the PhD Start-up Fund of TYUST(20132013)

摘　　要：研究采用4种DNA序列,分析了各片段序列特征以及在串珠藻科植物中种属水平的鉴定能力,包括线粒体COI-5P、cox2-3 spacer序列,以及叶绿体rbc L、UPA序列。结果表明,COI-5P、cox2-3 spacer、UPA以及rbc L序列的PCR扩增成功率分别为96%、100%、96%和98%。其中,COI-5P、cox2-3 spacer和UPA的片段大小符合标准DNA条形码的判定标准,即片段大小在300—800 bp,能够通过单对引物双向测序获得。系统发育分析的结果显示,这4种DNA片段在串珠藻属植物的鉴定中能够鉴定大部分的种类,但COI-5P、cox2-3 spacer以及rbc L序列均不能将两种中国特有种洪洞串珠藻B.hongdongense和长柄串珠藻B.longipedicellatum与弧形串珠藻B.arcuatum分开。在种水平的鉴定中,UPA基因的种间差异最大,显示了较好的分离效果,在串珠藻科植物的鉴定中可以作为一个标准的DNA条形码。DNA barcoding refers to the application of a small number of DNA fragments to achieve reliable,automatable species-level identification. In this study, the suitability of four candidate sequence regions wereassessed-mitochondrial COI-5P and cox2-3 spacer, plastid rbcL and UPA-for species delimitation and discrimination in family Batrachospermaceae. The percentage of successful PCR amplifications of COI-5P,cox2-3 spacer, UPA, and rbcL markers was 96%, 100%, 96%, and 98%, respectively. COI-5P, UPA, andcox2-3 spacer sequence lengths were amenable to the acquisition of bidirectional sequencing reads usingsingle primer pairs and met our size criterion of 300-800 bp. Phylogenetic analyses revealed that all four sequence regions were useful for species-level identification in the genus Batrachospermum except for some allied species. The two Chinese endemic species B. hongdongense and B. longipedicellatum were unable to differentiate from B. arcuatum using COI-5P, cox2-3 spacer, and rbcL markers, excepted for the UPA region.For species-level identification, the UPA locus exhibited the highest interspecific distances. We therefore recommended the plastid UPA gene as a standard DNA barcode in Batrachospermaceae, but acknowledge thatthere are no shared alleles between the endemic species.

关 键 词：DNA条形码 串珠藻科 分子系统发育 红藻门 

分 类 号：Q949.29[生物学—植物学]