2015年广西地区PRRSV流行株ORF5和Nsp2基因分子流行病学调查  

An Nsp2 deletion identified based on molecular epidemiology of the porcine reproductive and respiratory syndrome virus in Guangxi Province in 2015

作　　者：孙文超[1,2] 张萍[2,3] 解长占 张金勇[2,3] 曹亮[2,3] 南福龙 韩继成[2] 温树波[2] 肖朋朋[2] 张赫[2] 庄忻雨 靖杰[2,4] 崔卓栋 柴丹 鲁会军[2] 金宁一[2] 

机构地区：[1]广西大学动物科学技术学院,广西南宁5300041 [2]军事医学科学院军事兽医研究所,吉林长春130122 [3]吉林农业大学动物科学技术学院,吉林长春130118 [4]吉林大学动物医学学院,吉林长春130062

出　　处：《中国病原生物学杂志》2017年第3期197-200,共4页Journal of Pathogen Biology

基　　金：国家高技术研究与中国发展计划(863计划)项目(No.2011AA10A208)

摘　　要：目的对广西地区猪繁殖与呼吸综合征病毒(PRRSV)Nsp2和ORF5基因进行分子流行病学调查。方法采集广西地区猪病料样品45份,PCR扩增PRRSV毒株Nsp2和ORF5基因并进行遗传进化分析。结果 RT-PCR检测PRRSV 7份猪病料阳性。7株病毒与VR-2332和LV株的同源性分别为83.5%88.7%和62.1%64.8%。遗传进化分析显示,7株病毒分属于两个亚群,3株属于亚群Ⅳ,4株属于亚群Ⅵ。Nsp2序列分析显示,6株病毒有高致病性PRRSV 1+29aa氨基酸的缺失特征,其中GXBB11-2015在此基础上出现了新的20个氨基酸缺失,缺失碱基数为150个碱基。结论高致病性PRRSV已成为广西地区优势毒株,病毒Nsp2基因新的碱基缺失是PRRSV变异的又一证据。Objective To investigate the molecular epidemiology of the Nsp2 and ORF5 genes of the porcine reproduc tive and respiratory syndrome virus （PRRSV） found in Guangxi. Methods In total, 45 samples of PRRSV were col- lected from Guangxi in 2015, and the Nsp2 and ORF5 genes of 7 strains were used to determine their molecular epidemiology. Results The 7 samples tested positive for PRRSV according to RT-PCR. The 7 strains were 83. 5%-88. 7% similar to VR-2332 and 62.1 $- 64.8% similar to I.V. The phylogenetic tree indicated that the 7 strains could be divided into 2 subgroups. Three strains were clustered in subgroup IV while the other 4 strains were clustered in subgroup VI. Sequencing of Nsp2 indicated that 6 strains had deletion of amino acid 29, which was similar to highly pathogenic PRRSV 1. The Nsp2 gene of GXBB11-2015 was found to have deletion of 20 amino acids and deletion of 150 bases. Conclusion Highly pathogenic PRRSV has become the predominant strain in Guangxi. Detection of deleted bases in Nsp2 provided new evidence of PRRSV mutations, laying a foundation for further study of the effect of gene deletion on pathogenicity.

关 键 词：猪繁殖与呼吸综合征 ORF5基因 Nsp2基因缺失 

分 类 号：R37[医药卫生—病原生物学]