尘螨变应原单克隆抗体的制备及应用研究进展  

Progress in preparation of monoclonal antibodies against dust mite allergens and their use

作　　者：孙金霞[1] 滕飞翔[1] 崔玉宝[2] 

机构地区：[1]盐城卫生职业技术学院医学技术学院,江苏盐城224005 [2]盐城市第三人民医院中心实验室,江苏盐城224001

出　　处：《中国病原生物学杂志》2017年第3期I0002-I0004,F0003,F0004,共5页Journal of Pathogen Biology

基　　金：国家自然科学基金项目(No.NSFC31272369;NSFC81001330;30660166);盐城市医学科技发展计划(No.YK2015054);江苏省卫生职业院校文化建设课题(No.JCH201506);江苏省大学生创新创业计划(No.201612682006Y)

摘　　要：单克隆抗体是B淋巴细胞和骨髓瘤细胞杂交形成的杂交瘤细胞形成的,其具有高度的特异性。变应原是诱导机体产生特异性IgE的抗原性物质。尘螨是室内变应原的重要来源物质。本文综述了单克隆抗体在变应原的定量、纯化和鉴定,筛选变应原表位,变应原定位研究以及在过敏性疾病治疗中的应用,并对发展前景进行展望。Monoclonal antibodies are cells. Monoclonal antibodies have a high produced by hybridoma cells produced by fusing B lymphocytes and myeloma degree of specificity. An allergen is an antigenic substance that can induce the body to produce specific IgE antibodies. Dust mites are a key cause of anaphylactic disorders such as allergic asthma, rhinitis, and dermatitis. This review describes advances in the study of monoclonal antibodies against dust mite allergens. This paper also describes the use of those monoclonal antibodies in allergen quantification, purification and identification of environmental allergens, screening of allergens for epitopes, locating allergens, and the treatment of allergic diseases.

关 键 词：尘螨变应原 单克隆抗体 应用 综述 

分 类 号：R384.4[医药卫生—医学寄生虫学]