益气活血方对关节炎大鼠滑膜组织IL-8、NF-κB的影响  

Effect of Yiqi Huoxue Prescription on IL-8 and NF-κB of Synovial Tissue of Adjuvant Arthritis Rats

作　　者：陈利锋[1] 丰瑞兵 王华松[3] 陈芳[4] 王丽平[4] 刘琴[4] 王俊伟[2] 王庆伟[2] 邓超[2] 

机构地区：[1]中国人民解放军武汉总医院中西医结合科,湖北武汉430070 [2]湖北中医药大学针灸骨伤学院,湖北武汉430061 [3]中国人民解放军武汉总医院骨科,湖北武汉430070 [4]中国人民解放军武汉总医院医学实验科,湖北武汉430070

出　　处：《湖北中医药大学学报》2017年第2期4-8,共5页Journal of Hubei University of Chinese Medicine

基　　金：中国博士后科学基金(项目编号:2015M582905);湖北省卫计委中医药项目(项目编号:2013Z-Y37);湖北省自然科学基金(项目编号:2016CFB649)

摘　　要：目的观察益气活血方对佐剂型关节炎大鼠滑膜组织IL-8、NF-κB的影响。方法随机将60只Wistar大鼠分为空白组、模型组、益气活血方高剂量组(高剂量组)、益气活血方中剂量组(中剂量组)、益气活血方低剂量组(低剂量组)、雷公藤组。除空白组外,其余大鼠通过弗氏完全佐剂诱导建立佐剂型关节炎模型。造模两周后给药,高、中、低剂量组分别给予浓度为2、1、0.5g/mL益气活血方汤剂,雷公藤组给予0.94mg/mL雷公藤多苷片水溶液,空白组和模型组给予蒸馏水10mL/kg,每日1次,持续3周。检测大鼠关节炎症指数、大鼠滑膜组织病理组织学改变及大鼠滑膜组织中IL-8、NF-κB的表达水平。结果灌胃3周后,各给药组在以上各检测指标上较模型组均有显著改变(P<0.01),益气活血方高剂量组的治疗作用优于低剂量组与雷公藤组(P<0.05)。结论益气活血方能改善佐剂型关节炎大鼠的关节炎症表现,其作用机制可能与抑制滑膜组织中IL-8、NF-ΚB的表达有关。Objective To explore the effect of Yiqi Huoxue prescription on IL - 8 and NF - κB of synovial tissue of adjuvant arthritis（AA） rats. Methods 60 Wistar rats were randomly divided into control group, model group, Tripterygium Glycosides tablets group （TGT） group, YQHXP high dose group （YQHXP -H）, YQHXP middle dose group （YQHXP - M） and YQHXP low dose group（ YQHXP - L）. Except for the control group, each group was induced by Complete Freund＇ s Adjuvant（CFA） to establish AA rat model. Two weeks after the first modeling, each drug group was given medicine accordingly by gastric perfusion once a day for 3 weeks. Control group and model group were given isopyknie normal saline in the same way. Arthritis index, histopathological changes of synovial tissue and the expression levels of IL - 8 and NF -κB of synoviat tissue were main outcome measures. Results Three weeks after treatment, these factors were significantly changed in the drug treated group compared with in model group （P 〈 0. 01 ）. The therapeutic effect of YQHXP - H group was obviously better than that of YQHXP - L group and TGT group （P 〈 0.05 ）. Conclusion The results indicated that YQHXP exert an therapeutic effect on the AA rat model, and its therapeutic mechanism may be associated with its inhibition in the expression of IL -8 and NF -κBof synovial tissue of AA rats.

关 键 词：类风湿性关节炎 滑膜组织 白细胞介素8 NF-ΚB 

分 类 号：R285.5[医药卫生—中药学]