牛黄参含药血清调控Bax、Bcl-2表达诱导人肝癌细胞HepG-2凋亡的实验研究  

Research on the Regulation of the Expression of Bax and Bcl-2 in Human Hepatoma Cell Line HepG-2 Induced by the Serum Containing Niuhuangshen Capsule

作　　者：金伟伟[1,2] 白山岭[1] 肖明中[2] 陈晨[1,2] 刘建忠[2] 胡爱萍[1] 赵映前[1] 

机构地区：[1]湖北中医药大学,湖北武汉430065 [2]湖北省中医院,湖北武汉430061

出　　处：《湖北中医药大学学报》2017年第2期11-14,共4页Journal of Hubei University of Chinese Medicine

基　　金：湖北省自然科学基金项目(项目编号:2011cdb464;2010cd807103)

摘　　要：目的观察牛黄参胶囊含药血清对人肝癌细胞HepG-2的Bax、Bcl-2的调控作用。方法建立并分为空白对照组,环磷酰胺组和牛黄参胶囊5%、10%和20%含药血清组。采用实时定量PCR检测Bax、Bcl-2 mRNA的表达水平;免疫组织化学法检测HepG-2细胞Bax、Bcl-2的表达;Western blotting检测Bcl-2蛋白的表达水平。结果牛黄参胶囊20%含药血清能在基因和蛋白水平明显上调Bax表达(P<0.01),明显抑制Bcl-2的表达(P<0.01),并具有明显的剂量依赖性。结论牛黄参胶囊含药血清能明显上调Bax表达,同时下调Bcl-2表达,可能是其诱导肝细胞凋亡的机制之一。Objective To investigate the effect of the serum containing Niuhuangshen capsule （NHS） on the regu- lation of Bax and Bcl - 2 in HepG - 2. Methods The effects of the NHS on the regulation of Bax and Bcl - 2 in human hepatoma carcinoma HepG -2 were determined by real time PCR in mRNA level and the Bax/Bel -2 protein expression were evaluated by immunohistochemistry and western blotting. Results The expression of Bax was increased with dose dependent （P 〈0.01 ） in 48h, and the expression of Bcl- 2 was decreased with dose dependent（P 〈 0.01 ） in RNA and protein level. Conclusion The serum containing Niuhuangshen capsule probably induces HepG -2 apoptosis by taking advantage of up - regulation Bax and down - regulation Bcl - 2.

关 键 词：HEPG-2细胞 凋亡 牛黄参胶囊含药血清 BAX BCL-2 

分 类 号：R285[医药卫生—中药学]