大孔树脂分离红花中羟基红花黄色素A的优化工艺  

Optimization of Separating Conditions for Hydroxysafflor Yellow A by Macroporous Resin Column Chromatography

作　　者：唐红[1] 谭沙[1] 吴栋[1] 

机构地区：[1]铜仁学院材料与化学工程学院,贵州铜仁554300

出　　处：《广州化工》2017年第8期49-52,共4页GuangZhou Chemical Industry

摘　　要：探讨大孔树脂分离羟基红花黄色素A的最优工艺。以菊科红花为原料,采用微波提取法,以静态吸附和解吸附量为参考指标,考察D4020、X-5、DM301、AB-8四种大孔树脂对红花中羟基红花黄色素A的分离性能及最佳洗脱浓度及pH值,在动态吸附单因素试验的基础上采用正交试验优化分离条件,采用紫外可见分光光度法对羟基红花黄色素A含量进行定量分析。研究表明:X-5树脂对红花中羟基红花黄色素A具有较好吸附和解吸附性能;最优分离条件为:流速为1.5 mL/min,径高比为1∶10,上样量为柱体积的3%。此方法简单可行,重现性好。The optimal conditions for Hydroxysafflor Yellow A （HSYA） by Macroporous Resin Column Chromatography （MRCC） were explored. Using HSYA as raw material, microwave as extraction method, static adsorption and desorption ratio as observation index, four MRCCs of D4020, X-5, DM301 and AB-8 were used to study optimal separating capacity, solvent concentration and pH of HSYA in Carthamus tinctorius L. The orthogonal test optimal separating conditions were adopted on the basis of the dynamic adsorption single factor test, and the value of the extraction HSYA was evaluated by ultraviolet-visible spectrophotometry （UV）. The result showed that the optimal process condition was as follows： X-5 MRCC showed better adsorption and desorption properties for HSYA, the flow speed was 1.5 mL/min, diameter-to-height ratio was 1 ： 10, upper quantity of column volume was 3%. So the method was simple and feasible, and had good reproducibility.

关 键 词：大孔树脂 菊科红花 分离 羟基红花黄色素A 

分 类 号：R932[医药卫生—生药学]