北京西山地区长角血蜱及其体内伯氏疏螺旋体实验观察  

Morphological Observation of Haemaphysalis congicornis and Borrelia Burgdorferi in the Tick's Body in Xishan Mountain in Beijing

作　　者：安京媛[1] 周国萍[2] 姚桂玲[1] 艾承绪[3] 李全民[1] 

机构地区：[1]武警总医院,北京100039 [2]北京军区总医院 [3]军事医学科学院微生物流行病研究所

出　　处：《中国媒介生物学及控制杂志》2002年第4期282-284,T001,共4页Chinese Journal of Vector Biology and Control

摘　　要：目的 :实验观察长角血蜱及其体内伯氏疏螺旋体形态结构。方法 :1994～ 1996年在北京西山地区采集长角血蜱 180只。标本应用蜱整体封片法、冰冻切片和石蜡切片结合HE染色和硝酸银染色法分别检测和光镜观察。结果 :蜱整体封片法直接镜检即可观察到蜱外部基本形态结构。HE染色观察蜱内部器官细微形态结构。冰冻切片和石蜡切片结合硝酸银染色 ,检出北京西山地区长角血蜱 (包括幼蜱、若蜱、成蜱和饱血蜱 )伯氏疏螺旋体感染率为 2 3 % ,显微镜下可清晰观察到蜱体伯氏疏螺旋体多种不同的形态。结论Objective:To observe the morpholosis of Haemaphysalis longicornis and histomorphological features of Borrelia burgdorferi (BB). Methods: Morpholosis of ticks may be observed by mounting method under light microscope. Morphology of the tick was observed by HE staining. Histomorphological features differentialy and morphology of BB was observed by frozed section and paraffin section combinend with sliver nitrate staining.Results:The Haemaphysalis longicornis were collected from 1994 to 1996 in Xishan Mountain in Beijing. 180 individuals were examined. The infection rate of BB in ticks was 23% in the region. Conclusion:Three methods is useful for study and epidemic inspection of victor and BB of Lyme disease.

关 键 词：北京西山地区 长角血蜱 实验观察 莱姆病 伯氏疏螺旋体 

分 类 号：R377[医药卫生—病原生物学] R384.4[医药卫生—基础医学]