滇重楼内生真菌多样性分析  

Diversity research of endophytic fungi from Paris polyphylla var. yunnanensis

作　　者：段荣婷[1] 李洪涛[1] 李红玉[1] 周皓[1] 杨雪琼[1] 蔡乐[1] 杨亚滨[1] 

机构地区：[1]云南大学化学科学与工程学院,云南昆明650091

出　　处：《中国医药导报》2017年第9期12-15,共4页China Medical Herald

基　　金：国家自然科学基金资助项目(81560571);云南省教育厅科学研究基金资助性项目(2016ZZX003)

摘　　要：目的研究滇重楼内生真菌多样性。方法本实验对云南弥勒和临沧野生滇重楼内生真菌进行分离,并基于16S r DNA序列分析和形态学方法进行鉴定。结果从两地滇重楼中共分离得到42株内生真菌,分别归属于16个属。其中弥勒滇重楼归属于13个属,而临沧滇重楼归属于8个属。结论滇重楼内生真菌的差异性可能与宿主生存环境及分离部位的结构和成分等因素有关。Objective To investigate the diversity of endophytic fungi from Paris polyphylla var. yunnanensis. Methods The endophytic fungi were isolated from wild Paris polyphylla collected from Mile and Lincang of Yun＇nan Province, and identified by 16S rDNA sequence analysis and morphological method. Results 42 strains of endophytic fungi were classified into 16 genus. Meanwhile, 13 genus and 8 genus endophytic fungi were isolated from Paris polyphyUa of Mile and Lincang respectivelY. Conclusion It is reasonable to propose that the differences of endophytic fungi are related to different habitat, constituents and isolating positions of the host.

关 键 词：滇重楼 内生真菌 分离 鉴定 多样性 

分 类 号：R939.5[医药卫生—生药学]