基于对曙红Y和罗丹明B之间的能量转移反应的荧光光谱法测定痕量锰  

Fluorescent Spectroscopy Determination of Trace Amounts of Manganese Based on the Energy Transfer Between Eosin Y and Rhodamine B

作　　者：崔英[1] 张楠[1] 

机构地区：[1]河南科技学院资源与环境学院,新乡453003

出　　处：《理化检验（化学分册）》2017年第4期429-431,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：河南省教育厅科学技术研究重点项目(14A610014;13A610514);河南省科技厅基础与前沿研究项目(132300410139)

摘　　要：在pH 5.5的乙酸盐缓冲溶液中,并在十二烷基苯磺酸钠的存在下,曙红Y与罗丹明B之间发生能量转移,导致罗丹明B的荧光增强;而在40℃温度条件下,当有锰(Ⅱ)存在时,罗丹明B在波长576nm处的荧光发射发生猝灭现象。进一步试验表明,当在激发波长为508nm和发射波长为576nm的条件下,上述荧光猝灭的强度ΔF,即试液中无锰(Ⅱ)及有锰(Ⅱ)存在时所测得的荧光强度F1及F2之差,与锰(Ⅱ)的浓度在1.0×10-8~2.0×10-7 mol·L^(-1)范围内呈线性关系,检出限(3s/k)为4.0×10-9 mol·L^(-1)。据此提出了测定样品中痕量锰(Ⅱ)的荧光光谱法。应用此方法对同一试样中痕量锰作了测定,并以此样品作基体,用标准加入法进行回收试验,测得回收率为106%,样品测定值的相对标准偏差(n=11)为4.8%。In an acetate buffer solution of pH 5.5 and in the presence of sodium dodecyl benzene sulfonate, enhancement of fluorescence intensity of rhodamine B was observed due to the occurrence of energy transfer between eosin Y and rhodamine B. It was shown further that when under a temperature of 40 ℃ and in the presence of trace amounts of Mn（ II ）, the fluorescence of rhodamine B emilted at 576 nm was quenched and that linear relationship was obtained between the magnitude of the fluorescence quenching, △F [which is the difference of fluorescence intensity of the test solution without Mn（ Ⅱ ）, F1 and of that with Mn（ 11 ）, F2 measured at ;rex =508 nm and λem = 576 nm] and the concentration of Mn（ Ⅱ ） ion in the range of 1.0 × 10^-8 --2.0 × 10^-7 mol · L^-1, with detection limit （3s/k） of 4. 0×10^- 9mol · L^-1. Based on these facts, a method of fluorescent spectroscopy determination of trace amount of Mn（ Ⅱ） in samples was proposed. Tests for precision and recovery （by standard addition method） were performed by analyzing a substantial sample with the proposed method, giving values of RSD （n= 11） of 4. 8 % and of recovery of 106%.

关 键 词：荧光光谱法 能量转移 曙红Y 罗丹明B 荧光猝灭 痕量锰(Ⅱ) 

分 类 号：O657.34[理学—分析化学]