商陆不同炮制品对大鼠的利尿作用及其对睾丸、肾脏水通道蛋白的调节作用  

Effect of Different Processed Products of Phytolaccae Radix on Diuretic Activity and Regulation Aquaporins of Testicles and Kidneys in Rats

作　　者：薛非非 张朔生[1] 马俊楠[1] 孟祥龙[1] 王明芳[1] 何美菁 

机构地区：[1]山西中医学院,山西晋中030619

出　　处：《中国实验方剂学杂志》2017年第9期1-5,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81173555)

摘　　要：目的:研究商陆生品及不同炮制品的利尿作用,初步探究其利尿作用机制。方法:采用水负荷大鼠模型,将80只SD大鼠随机分为空白组、模型组、商陆皂苷甲(Es A)组、生品组、不同炮制品(醋炙、醋煮、水煮、清蒸)组,每组10只。同时进行正常状态大鼠利尿实验,将70只SD大鼠随机分为空白组,Es A组,生品组及不同炮制品(醋炙、醋煮、水煮、清蒸)组,每组10只。各给药组的剂量分别为0.005,1.8,1.8,1.8,1.8,1.8 g·kg^(-1),通过给予水负荷模型大鼠及正常大鼠不同的药物,分别测定排尿量,并进行水负荷大鼠大鼠睾丸水通道蛋白9(AQP9)因子表达、肾组织AQP2和AQP4因子的表达试验及正常大鼠睾丸AQP9因子的表达试验。结果:综合6 h总尿量,Es A,商陆生品及不同炮制品均对水负荷大鼠表现出利尿作用;对于正常大鼠仅醋煮品表现出利尿作用。聚合酶链式反应(PCR)技术中对于水负荷大鼠,AQP2 mRNA表达均显著降低,AQP4和AQP9 mRNA表达大部分无显著变化;对于正常大鼠,醋煮组AQP9 mRNA表达显著降低,其余各组无显著变化。结论:商陆对于水负荷大鼠表现出利尿作用,但对正常大鼠利尿作用不明显;商陆的利尿作用可能与AQP2和AQP9 mRNA表达下降有关。Objective： To evaluate the diuretic effect of different processed products of Phytolaccae Radix and explore its mechanism of diuretic action. Method： A water load rat model was used,eighty SD rats were randomly divided into the blank group, model group, esculentoside A（ Es A） group, raw product group and different processed products（ vinegar broiled,vinegar boiled,boiled and steamed） group,10 rats in each group.At the same time, SD rats were randomly divided into the blank group, EsA group, raw product group and different processed products（ vinegar broiled,vinegar boiled,boiled and steamed） group,10 rats in each group.The urine output was observed and relative mRNA expression of aquaporin-9（ AQP9） of testicles, AQP2 and AQP4 of kidneys were determined. Result： In this respect of total urine output in 6 h,EsA,raw product group and different processed products of Phytolaccae Radix all showed a diuretic effect on water load rats. For normal rats,only vinegar-boiled product showed diuretic effect. PCR experiments showed that for water load rats,AQP2 mRNA expression were significantly decreased,AQP4 and AQP9 mRNA expression did not change significantly.For normal rats,AQP9 mRNA expression in vinegar-boiled group was significantly decreased,the other groups did not change significantly. Conclusion： Phytolaccae Radix for water load rats shows a diuretic effect, but it is unapparent for normal rats. Diuretic effect of Phytolaccae Radix may be related to the decrease of AQP2 and AQP9 mRNA expression.

关 键 词：商陆皂苷甲 商陆 炮制品 利尿作用 水通道蛋白 醋炙 

分 类 号：R283.1[医药卫生—中药学] R284.1[医药卫生—中医学]