人中黄炮制前后6种化学成分的含量变化及其质量评价  

Content Changes of Six Chemical Constituents in Manure-prepared Licorice Before and After Being Processed and Its Quality Evaluation

作　　者：王胜超[1] 张振凌[1] 刘艳[1] 韩宁宁[1] 李柯柯[1] 黄亚森[1] 

机构地区：[1]河南中医药大学,郑州450008

出　　处：《中国实验方剂学杂志》2017年第9期11-16,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家中医药管理局中药炮制技术传承基地建设项目[2100601中医(民族医)专项];河南中医药大学研究生科研苗圃工程项目(MPYJS2017-06)

摘　　要：目的:建立同时测定人中黄中甘草苷、甘草素、异甘草素、甘草酸、甘草查尔酮A及甘草次酸6种化学成分含量的方法,比较甘草转化成人中黄前后三萜类和黄酮类成分的变化情况,评价人中黄样品的质量,为建立其科学制备方法提供参考。方法:采用HPLC,流动相乙腈-0.05%磷酸水溶液梯度洗脱,采集时间100 min,流速1 m L·min^(-1),柱温30℃,检测波长237 nm。采用聚类分析、主成分分析和G检验3种方法进行分析。结果:12批市售人中黄样品中甘草苷,甘草素,异甘草素,甘草查尔酮A,甘草酸和甘草次酸的质量分数分别为0.04~0.23,0.25~0.66,0.17~0.37,0.33~3.42,1.54~15.21,0.03~0.95 mg·g^(-1)。甘草中这6种成分的平均质量分数分别为1.64,0.32,0.17,0.50,22.46,0 mg·g^(-1)。相比甘草,市售人中黄中甘草苷含量下降了98.17%,甘草素升高了40.63%,异甘草素升高了17.65%,甘草酸下降了76.80%,甘草查尔酮A升高了68.00%。甘草与人中黄具有本质差别,不同方法制备的人中黄及12批市售样品间均具有一定差异。结论:该方法稳定可行、重复性好。当前市面流通的人中黄样品质量参差不齐、差异明显,可能是由于人中黄的制备工艺存在差异或原料甘草品种不同所致,且在某种程度上氨化环境对于人中黄的形成具有积极作用。Objective： To establish an accurate HPLC method for the determination of 6 components （liquiritin,liquiritigenin,isoliquritigenin,licochalcone A,glycyrrhizic acid and glycyrrhetinic acid） in manure-prepared licorice.To compare content changes of triterpenes and flavonoids in manure-prepared licorice before and after being processed and evaluate its quality. Method： The analyses were performed on a Agilent Exlipse C18 column （4.6 mm×250 mm,5 μm） with mobile phase of acetonitrile-0.05% phosphoric acid aqueous solution for gradient elution at a flow rate of 1.0 mL·min^-1,the detection wavelength was set at 237 nm and the column temperature was at 30℃.Quality of manure-prepared licorice was analyzed by cluster analysis,principal component analysis and G-test. Result： The contents of these 6 components in 12 batches of manure-prepared licorice samples were in the ranges of 0.04-0.23,0.25-0.66,0.17-0.37,0.33-3.42,1.54-15.21,0.03-0.95 mg·g^-1,respectively;and the average contents of these 6 components in Glycyrrhizae Radix et Rhizoma were 1.64,0.32,0.17,0.50,22.46,0 mg·g^-1,respectively.Glycyrrhizae Radix et Rhizoma and manure-prepared licorice had essential difference,there were some differences among samples prepared by different methods and 12 batches of commercial samples. Conclusion： The method is simple and reproducible.Quality of manure-prepared licorice is influenced by preparation process,raw materials and ammoniated environment.

关 键 词：人中黄 主成分分析 聚类分析 甘草苷 甘草查尔酮A 甘草酸 甘草 

分 类 号：R283.4[医药卫生—中药学] R284.1[医药卫生—中医学]