葫芦茶叶抗过敏性哮喘组分分析  

Analysis of Anti-allergic Asthma Constituents in Tadehagi triquetrum Leaves

作　　者：丁辉[1] 史丽颖[1,2] 陈瑶[1] 卢轩[1,2] 冯宝民[1,2] 王永奇[2] 于大永[1,2] 

机构地区：[1]大连大学生命科学与技术学院,辽宁大连116622 [2]大连大学药物研究所,辽宁大连116622

出　　处：《中国实验方剂学杂志》2017年第9期30-35,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81073012);辽宁省高等学校杰出青年学者成长计划项目(LJQ2013134)

摘　　要：目的:研究葫芦茶抗过敏性哮喘的活性组分及化学成分。方法:采用中、高压液相色谱法对葫芦茶叶提取物进行多组分富集,采用无标记整合药理学方法筛选富集得到的组分对GPR35受体的激动活性,应用HPLC-TOF-MS技术对活性组分的化学成分进行分析。结果:葫芦茶叶的95%乙醇提取物经正庚烷-70%甲醇萃取,70%甲醇萃取层经C18中压制备柱,分别以5%,70%和95%的甲醇洗脱,其中70%甲醇洗脱部分经XAmide高压制备色谱柱,以甲醇-水洗脱,按色谱峰收集流分,共得到10个组分。GPR35受体激动活性筛选结果显示组分Fr4,Fr5,Fr6,Fr7,Fr9,Fr10对GPR35受体有较强的激动活性,同时对GPR35受体激动剂敏喘宁显示了脱敏活性,由此推测上述组分为葫芦茶抗过敏性哮喘的活性组分。采用HPLC-TOF-MS技术对上述活性组分的化学成分进行了分析,从中鉴定出乌索酸,儿茶素,山柰酚-3-O-α-鼠李糖基(1→6)-β-葡萄糖苷,槲皮素-3-O-α-鼠李糖基(1→6)-β-葡萄糖苷/槲皮素-3-O-α-鼠李糖基(1→6)-β-半乳糖苷,间苯三酚-1-O-β-葡萄糖苷,槲皮素-3-O-β-葡萄糖苷,山柰酚-3-O-β-葡萄糖苷,山柰酚-3-O-β-半乳糖苷,triquetrumone E,triquetrumone F,山柰酚,胡萝卜苷,芹菜素,共12个化合物。结论:组分Fr4,Fr5,Fr6,Fr7,Fr9,Fr10为葫芦茶抗过敏性哮喘的有效组分,从活性组分中检测到的12个化合物可能为葫芦茶抗过敏性哮喘的有效成分。Objective： To investigate the anti-allergic asthma constituents in Tadehagi triquetrum.Method： The fractions were enriched from T. triquerum by using medium or high pressure liquid chromatography;such active fractions as G protein-coupled receptor-35（ GPR35） agonists were screened out by using label-free dynamic mass redistribution assays; HPLC-TOF-MS was used to analyze the main components in active fractions.Result： The 95% ethanol extracts of leaves of T. triquerum were extracted with n-heptane-70% methanol; 70%methanol extraction layer was eluted with 5%,70% and 95% methanol through C_（18） medium-pressure preparation column; among them,the part eluted with 70% methanol was eluted with methanol-water through XAmide highpressure preparative chromatography column,and separated into 10 components based on chromatographic peaks.The 10 fractions were enriched from T. triquerum; fraction 4,5,6,7,9 and 10 exhibited strong activities against GPR35 receptor,suggesting the above components are anti-allergic asthma constituents of T. triquerum. HPLCTOF-MS was used to analyze the chemical constituents of the above components and identify 12 constituents,namely ursonic acid,catechinic acid,kaempferol-3-O-α-rhamanopyranosyl（ 1 →6）-β-glucoside,quercetin-3-O-α-rhamanopyranosyl（ 1 → 6）-β-glucoside / quercetin-3-O-α-rhamanopyranosyl（ 1 → 6）-β-galactoside,phloroglucinol-3-O-β-galactoside,triquetrumone E, triquetrumone F, kaempferol, daucosterol and apigenin.Conclusion： Fraction 4,5,6,7,9 and 10 are active fractions,and the compounds identified from active fractions might be anti-allergic asthma constituents.

关 键 词：葫芦茶 抗过敏性 哮喘 GPR35 成分分析 

分 类 号：R284.1[医药卫生—中药学]