固相萃取结合超高效液相色谱质谱方法同时测定尿中的6种苯丙胺类毒品（英文）  

Simultaneous Determination of Six Amphetamines in Urine by Solid Phase Extraction and UPLC-MS/MS

作　　者：王朝虹[1] 张琳[1] 赵蒙[1] 李虹 刘帅[3] 

机构地区：[1]最高人民检察院检察技术信息研究中心,北京100040 [2]公安部毒品分析及禁毒技术重点实验室,昆明650000 [3]昆明医科大学法医学院,昆明650000

出　　处：《刑事技术》2017年第2期111-115,共5页Forensic Science and Technology

基　　金：Ministry of Public Security's basic project exclusive for reinforcing police with science and technology(No.2016GABJC50);Fundamental research fund of Supreme People's Procuratorate(No.JBKY20150604)

摘　　要：本文建立了固相萃取-超高效液相色谱-质谱法(SPE-UPLC-MS/MS)同时测定尿中6种苯丙胺类毒品。尿样经过Oasis MCX固相萃取柱提取净化,应用电喷雾离子源(ESI)、多反应监测(MRM)正离子模式质谱进行定性及定量检验。6种苯丙胺类毒品在0.1~20 ng/mL范围内线性关系良好,相关系数优于0.9987,提取回收率大于78%,RSD小于10%。本方法简单、快速、灵敏、准确、可应用于尿液中痕量苯丙胺类毒品的分析。A rapid and sensitive method has been developed for simultaneous determination of 6 amphetamine-type drugs in urine sample by solid phase extraction-ultra performance liquid chromatography coupled with mass spectrometry（SPE-UPLC-MS/MS）.The samples were extracted with Oasis MCX solid phase extraction cartridge and analyzed with the UPLC-MS/MS system coupled with an electrospray ionization（ESI） source,finally having been measured in ESI＋ mode and multiple reaction monitoring（MRM）.Six amphetamine-type drugs were linear in the range of 0.1-20 ng/mL,with correlation coefficients over 0.9987.The recoveries were above 78%with RSD less than 10.0%.The method is simple,rapid,sensitive and accurate for the determination of trace amphetamine-type drugs in urine sample.

关 键 词：苯丙胺类 固相萃取 超高效液相色谱质谱 尿 

分 类 号：DF795.1[医药卫生—法医学]