积雪草总苷对糖尿病皮肤溃疡大鼠的促愈合作用研究  

Treatment of Chronic Ulcer in Diabetic Rats with Asiaticosides

作　　者：张丹丹[1] 张涵[1] 赵久凤[1] 石秀君[1] 杨建文[1,2] 聂绪强[1] 

机构地区：[1]遵义医学院药学院,贵州遵义563000 [2]遵义医学院附属医院,贵州遵义563003

出　　处：《中国药学杂志》2017年第8期643-648,共6页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助项目(81560712);贵州省联合基金(黔科合LH字[2014]7566号)资助项目;贵州省重大专项(黔科合重大专项字[2015]6010)资助项目;贵州省药剂学研究生卓越人才培养计划(黔教研合ZYRC字[2014]019)资助项目

摘　　要：目的本实验探讨积雪草总苷对大鼠糖尿病皮肤溃疡创面修复的影响。方法 38只雄性清洁级SD大鼠,随机分为正常组和模型组,代谢笼单笼饲养;模型组采用腹腔注射链脲佐菌素——全程皮肤缺损方法建立糖尿病皮肤溃疡(DCU)模型;模型建立后分别用低(22.29 mg·cm^(-2))、中(111.45 mg·cm^(-2))、高(222.93 mg·cm^(-2))剂量积雪苷霜软膏对DCU大鼠进行药物干预。治疗后3、7、10、14 d监测大鼠体重、血糖、饮水量及尿量;采用Adobe Photoshop CS5图像处理软件评价创面愈合率;利用HE染色观察DCU大鼠创面肉芽组织形态变化、上皮化、成纤维细胞增殖和血管新生。结果模型组大鼠体重、血糖与正常组比较有统计学差异(P<0.05);模型组大鼠尿量和饮水量均显著高于正常组;给药组创面愈合率高于模型对照组(P<0.01),以中剂量积雪苷霜软膏愈合效果最好(P<0.01);给药后创面肉芽组织生成增多,中剂量组上皮化较早及较高的成纤维细胞增殖,14 d表皮结构趋于完整。结论初步判断积雪草总苷对糖尿病皮肤溃疡大鼠创面具有促愈合作用。OBJECTIVE To investigate the treatment effects of chronic ulcer in diabetic rats with asiaticosides. METHODS Totally 38 male CL grade SD rats were randomly divided into normal group and model group, and were feed in metabolic cage separately. The low （22. 29 mg·cm^-2）, medium （ 111.45 mg·cm^-2 ） and high （222. 93 mg·cm^-2 ） dose of centella triterpenes cream were characterized and tested on excision ulcer wounds of streptozotocin-indnced diabetic rats. After treatment, the weight, blood glucose, water intake and total urine output were measured in 3, 7, 10 and 14 d respectively. The wound healing rate was evaluated by image processing software of Adobe Photoshop CS5. Histopathological studies were conducted on the healed wound tissues to observe for epi- thelisation, flbroblast proliferation and angiogenesis. RESULTS It was found that at all time points the areas of wounds treated with asiaticosides were smaller compared with the controls. The expression of weight, water intake, total urine output and blood glucose in- creased statistically in the model group （ P 〈 0. 05 ）. Pathological resuhs showed much better healing efficacy for the test samples com- pared with the control ones （ P 〈 0. 05）. The middle dose of eentella triterpenes cream was the best （P 〈 0. 01 ）, Histopathological studies had also shown that centella triterpenes cream treated wounds exhibited re-epithelisation, higher fibroblast proliferation, collagen synthesis, and angiogenesis. At 14 days post excision, the medium centella triterpenes cream treated group achieved complete repair. CONCLUSION These findings suggest that asiaticosides may have a potential application in the treatment of diabetic ulcers and chronic wound healing.

关 键 词：糖尿病 皮肤溃疡 愈合率 积雪草总苷 积雪苷霜软膏 病理学 

分 类 号：R965[医药卫生—药理学]