新型纳米级超声造影剂的制备及其特性  

Preparation and characteristics of novel nanoscaled ultrasound contrast agents

作　　者：段素娟[1] 时丹丹[1] 商蒙蒙 郭鲁[1] 孙霄[1] 孟冬[1] 李杰[1] 

机构地区：[1]山东大学齐鲁医院超声科,山东济南250012

出　　处：《山东大学学报（医学版）》2017年第4期50-54,共5页Journal of Shandong University:Health Sciences

摘　　要：目的制备新型纳米级脂质超声造影剂(UCAs),并对其基本特性及显影效果进行研究。方法采用机械振荡法制备脂质UCAs,针对制备过程中3个主要影响因素,设置正交试验,筛选最优制备条件,并在此条件下制备纳米级UCAs,检测其粒径、分散度、Zeta电位、稳定性及体外增强显影效果。结果正交试验结果显示,机械振荡时间对粒径影响有统计学意义(P<0.05),纳米级UCAs最优制备条件为:DPPA量为0.5 m L,机械振荡时间为90 s,离心时间为3 min;在此条件下制备出的纳米级UCAs平均粒径为(313.20±6.90)nm,分散度为0.15,Zeta电位为-12.40 m V,体外增强显影效果与声诺维相比差异无统计学意义(t=2.02,P>0.05)。4℃冰箱保存20 h后观察,该造影剂基本特性无明显改变。结论制备的纳米级UCAs粒径小,分散均一,性质稳定,具备良好的显影能力,且易于修饰,为进一步载药或构建纳米粒复合体等方面的研究奠定了基础。Objective To prepare lipid nanoscaled ultrasound contrast agents （UCAs）, and to explore their physio- chemical properties. Methods Orthogonal test was used to screen out the optimal condition for preparation aiming at three mainly factors. Amalgamator was applied to fabricate the lipid nanoscaled UCAs. The morphology, diameter, polydispersity index （P. I）, zeta potential and stability of nanoscaled UCAs were assessed. The enhancement effect of nanoscaled UCAs in vitro was also detected. Results The results of orthogonal test indicated that the oscillation time had a significant effect on the diameter of nanoscaled UCAs （ P 〈 0.05 ）. The optimal condition of preparation was as follows ： the amount of DPPA was 0.5 mL, oscillation time was 90 s, and centrifugation time was 3 min. The mean di- ameter and P. I of the nanoscaled UCAs fabricated under the optimal condition was （313.20±6.90） nm and 0.15, re- spectively. The Zeta potential was - 12.40 inV. There was no significant difference in the enhancement effect between the nanoscaled UCAs and SonoVue in vitro （ t = 2.02, P 〉 0.05 ）. The basic physical characteristics of the nanoscaled UCAs remained unchanged after being stored in 4 ℃ refrigerator for 20 hours. Conclusion Nanoscaled UCAs with smaller diameter and good stability were successfully fabricated. All these characteristics indicate a promising future in targeted molecular imaging and drug or gene delivery.

关 键 词：超声造影剂 纳米级 脂质 显影 纳米颗粒 

分 类 号：R445.1[医药卫生—影像医学与核医学]