金钱鱼雌激素受体erα、erβ1和erβ2克隆及表达分析  

Cloning and expression analysis of erα,erβ1 and erβ2 genes from Scatophagus argus

作　　者：李红[1] 张金鹏[1] 廉颖[1] 张俊彬[1] 

机构地区：[1]上海海洋大学水产与生命学院,上海201306

出　　处：《生物学杂志》2017年第2期40-45,共6页Journal of Biology

基　　金：国家自然科学基金委项目(31272662)

摘　　要：克隆到金钱鱼(Scatophagus argus)3种雌激素受体(erα,erβ1和erβ2)cDNA全长。其中,erα全长2621 bp,编码635个氨基酸;erβ1全长2891 bp,编码560个氨基酸;erβ2全长2410 bp,编码657个氨基酸。同源性分析表明,金钱鱼ERα,ERβ1和ERβ2与欧洲鲈(Dicentrarchus labrax),黑棘鲷(Acanthopagrus schlegelii),大口黑鲈(Micropterus salmoides)同源性最高,分别为91.7%、89.9%和88.4%。半定量结果显示,erα和erβ1主要在垂体、脑、性腺中表达,erβ2主要在肝脏、垂体、精巢中表达,其他组织表达量很低。就脑、垂体、性腺等组织而言,erα表达量高于erβ1和erβ2,肝脏中erβ2要高于erα。实时荧光定量结果表明,精巢中,erα在精子成熟时期显著升高,erβ1和erβ2则在精巢发育早期表达最高。卵巢中,金钱鱼雌激素受体主要在卵原细胞增长期和卵黄充满期表达。上述结果表明金钱鱼雌激素受体可能在精巢和卵巢发育中具有重要作用。Three estrogen receptors（ erα,erβ1 and erβ2） of Scatophagus argus were cloned in this study. The lengths of erα,erβ1and erβ2 were 2621 bp,2891 bp and 2410 bp,respectinvely,encoding of 635,560 and 657 arnino avids,corespondingly. The similarity analysis of amino acid sequences showed that ERα,ERβ1 and ERβ2 of Scatophagus argus shared the highest similarity with Dicentrarchus labrax,Acanthopagrus schlegelii and Micropterus salmoides,and the similarity was 91. 7%,89. 9% and 88. 4%,respectively. Semi-quantitative RT-PCR showed that ERα and ERβ1 were mainly expressed in hypophysis,brain and testis. The high expression of erβ2 was found in the liver,hypophysis and testis,while low expression was found in other tissues. erα expression was higher than erβ1 and erβ2 in these tissues of brain,hypophysis and gonad,but the erβ2 expression in liver was higher contrary to erα. Results of Real-time PCR showed that erα expression significantly increased in sperm maturation period,but erβ1 and erβ2 had highest expression in the early development in testis. In the ovary,estrogen receptor of Scatophagus argus was mainly expressed in oogonium and oocyte with full of yolk. These results demonstrated that the three estrogen receptors have important functions in the development of testis and ovary.

关 键 词：金钱鱼 雌激素受体 序列分析 基因表达 

分 类 号：Q78[生物学—分子生物学] S917.4[农业科学—水产科学]