星点设计-响应面分析法优化西芹叶中总黄酮的提取工艺  

Application of CCD-RSM for Optimization of Total Flavonoids Extraction in Celery Leaves

作　　者：王志波[1,2,3] 李成思[1,2,3] 张金魁[1,2,3] 林鹏程[1,2,3] 

机构地区：[1]青海民族大学药学院,西宁810007 [2]青海省青藏高原植物资源化学研究重点实验室,西宁810007 [3]青海省药物分析重点实验室,西宁810007

出　　处：《四川理工学院学报（自然科学版）》2017年第2期7-10,共4页Journal of Sichuan University of Science & Engineering(Natural Science Edition)

基　　金：青海民族大学创新项目(DCXM-2014-013)

摘　　要：以青海西芹叶为研究对象,通过星点设计-响应面分析法优化西芹叶中总黄酮的提取工艺,并以乙醇体积分数、提取时间、料液比为考察要素,进行三因素五水平星点试验设计,同时通过方差分析回归建立数学模型,响应面分析法优化西芹叶中总黄酮的提取工艺。结果显示,料液比和提取时间对西芹叶的总黄酮提取影响极其显著,最优提取工艺为提取时间50 min,乙醇体积分数50%,料液比1∶20(g/m L)。在此条件下,相对误差为1.24%,与模型预测值相符,总黄酮得率为18.36 mg/g。该工艺合理、快速,为今后西芹叶中总黄酮研究奠定基础。The extraction process of total flavonoids from celery leaves is optimized by CCD-RSM. The effects of ethanol concentration, extraction time, material liquid ratio are taken as the factors of investigation to lead three-factor five-level starpoint test design. Simultaneously, the mathematical model is established by regression analysis of variance, and the extraction process of total flavonoids from celery leaves is optimized by the Response Surface Methodology. The results show that materi- al liquid ratio and extraction time have significant effects on the extraction of flavonoids from celery leaves. And the optimum conditions are extraction time of 50 min, 50% ethanol concentration and material liquid ratio of 1：20 （g/mL）. The relative error of validation test is 1.24% , which is coincident with the predicted value. And the total yield of flavonoids was 18.36mg/g. The method is reasonable and rapid, which lays a foundation for the study of total flavonoids in celery leaves.

关 键 词：西芹叶 星点设计 响应面法 总黄酮 

分 类 号：TB115[理学—数学]