儿童麻疹疫苗接种后远期抗体水平及Th1/Th2细胞因子分泌特征  

Antibody levels and the secreting characterization of Th1/Th2 cytokines in the children long term after vaccination with measles vaccine

作　　者：汪培勤 汤菊妹 王健容 唐艳丽[1] 郭锦锦[2] 孙万邦[2] 

机构地区：[1]深圳市龙岗区妇幼保健院儿科,广东深圳518172 [2]遵义医学院珠海校区免疫学教研室,广东珠海519041

出　　处：《中国当代医药》2017年第11期4-8,共5页China Modern Medicine

基　　金：广东省深圳市龙岗区科技计划医疗卫生项目(201406063001042)

摘　　要：目的通过检测儿童接种后远期血清抗体水平和T细胞亚群分泌细胞因子,评价其体液免疫持久性及其对细胞免疫功能的影响。方法 2013年1月~2015年7月在深圳市龙岗区妇幼保健院儿童保健科体检的儿童,筛选麻疹疫苗接种的儿童32名,接种时间为3~10年,取外周血2 ml分离血清,用ELISA法检测麻疹抗体;根据麻疹抗体水平检测结果将所有受试者分组,即低应答组(9.00~19.99 IU/L)、中应答组(20.00~199.99 IU/L)和高应答组(200.00~320.00 IU/L);将麻疹疫苗接种的32例儿童血液标本分离的外周血单个核细胞(PBMC)进行培养,培养中设计不加麻疹疫苗的对照组、加疫苗刺激的实验组。用细胞因子微球检测技术检测细胞培养上清液中Th1细胞因子TNF-α和Th2细胞因子IL-10。结果抗体阳性率为81.2%,抗体水平为(115.03±95.20)IU/L;高应答组抗体水平显著高于中应答组和低应答组(P<0.05);实验组和对照组因子水平的中位数分别2021.81 pg/ml(TNF-α)、2566.93 pg/ml(IL-10)和1481.72 pg/ml(TNF-α)、1861.89 pg/ml(IL-10),实验组的TNF-α和IL-10水平显著高于对照组(P<0.05)。结论儿童麻疹疫苗接种后抗体阳性率较高,且接种后体内存在免疫记忆性T细胞,在接触抗原刺激后可介导细胞免疫应答。Objective To detect the serum antibody level and cytokine secretion of T cell subsets in children after vac- cination,and to evaluate the effect of humoral immunity and cellular immunity.Methods A total of 32 children with measles vaccination for 3-10 years were selected in Children＇s Health Care Department of Longgang District Maternal and Child Health Care Hospital of Shenzhen from January 2013 to July 2015. 2 ml blood samplewas used for measure measles antibody titers by ELISA from isolated serum,who were divided into low response group （9.00-19.99 IU/L）,mid- dle response group （20.00-199.99 IU/L） and high response group （200.00-320.00 IU/L） according to the results of measles antibody levels.The peripheral blood mononuclear cells （PBMC） were isolated from the other 3 ml blood sam- ple.The experimental group,stimulated by measles vaecine （3.0 μg/ml） and control group were set,without stimulation.Both groups were cultured and taken to detect TNF-α and IL-10by Cytometric Bead Array （CBA）.Results The positive rate of antibody was 81.2%,of which the level was （115.03＋95,20） IU/L.The antibody level in the high response group was sig- nificantly higher than those in the medium response group and low response group （P〈0.05）.The medians of factors in the experimental group and control group were respectively 2021.81 pg/ml （TNF-α）,2566.93 pg/ml （IL-10） and 1481.72 pg/ml （TNF-α）,1861.89 pg/ml （IL-10）,and the TNF-α and IL-10 levels in the experimental group were significantly higher than those in the control group （P〈0.05）.Conclusion Thepositive rate of antibody is high in children after vaccination with measles vaccine,and there are immune memory T cells,which can mediate cell-mediated im- mune response when exposure to antigen stimulation.

关 键 词：麻疹疫苗 抗体 细胞因子 远期免疫 免疫记忆 

分 类 号：R725.111[医药卫生—儿科]