UF-1000i尿有形成分分析仪、尿干化学分析与尿细菌培养的比较  

Comparison on the results of UF-1000i urine tangible component analyzer and urine dry chemical analysis and urine bacteria culture

作　　者：徐勇 

机构地区：[1]江苏省常州市金坛区中医医院检验科,213200

出　　处：《齐齐哈尔医学院学报》2017年第3期258-261,共4页Journal of Qiqihar Medical University

摘　　要：目的运用全自动尿液有形成分分析仪UF-1000i检测出的白细胞计数(WBC)、细菌定量计数(BACT)、细菌散点图和干化学法检测的亚硝酸盐反应(NIT)与尿液细菌培养结果的比对分析。方法用255人份样本做尿液干化学分析和有形成分分析,同时进行细菌培养及鉴定,对结果进行相关性分析。结果白细胞与细菌培养比对显示其具有较高的灵敏度为83%,但是其特异性为5.8%及误诊率为94.2%;细菌定量计数是拥有较高的灵敏度为97.2%和符合率为72.9%,但特异性为42%,应重视其参考价值,但不能作为诊断依据;细菌散点图应用能快速进行致病菌类别的判定,对合理使用药物有较好的参考价值;亚硝酸盐反应对尿路细菌感染的灵敏度为27.2%,其特异性很高为100%,一旦反应为阳性,基本判定有细菌感染的存在。结论尿液干化学分析项目的检测仍是目前诊断尿路感染的重要指标,尿液有形分析仪检测的细菌散点图是一种良好的判定致病菌的指标,但它们都存在一定的误诊率和漏诊率,而尿液细菌培养因报告时间较长,不能及时有效的为临床诊疗服务。了解其特点,联合应用能弥补单项指标的不足,从而提高泌尿道感染的诊断、治疗及疗效监测。Objective Use UF-1000 i urine tangible component analyzer to detect white blood cell count（ WBC）,quantitative count of bacteria（ BACT）,bacteria scattered point diagram in urine,and compared with the results of nitrite（ NIT） obtained from dry chemical method and the result of urine culture. Methods 255 urine samples were analyzed dry chemical analysis and tangible component analysis,at the same time,bacterial culture and identification was also carried out,the correlation of the results was analyzed.Results Compared with bacteria culture,it showed that the sensitivity was 83%,but its specificity was 5. 8% and the misdiagnosis rate was 94. 2%. The sensitivity of quantitative bacterial count was 97. 2% and coincidence rate was 72. 9%,but specificity was 42%,attention should be paid to the reference value,but not as a basis for diagnosis. Bacteria scattered point diagram can be used to quickly identify bacteria,and had a good reference value for the rational use of the drug. Sensitivity of nitrite reaction for bacterial urinary tract infection was 27. 2%,the specificity was high to 100%. Once the reaction is positive the determination of bacterial infection can be firmed.Conclusions Items of urine dry chemical analysis is still an important indicator in the diagnosis of urinary tract infection,the bacteria scattered point diagram is a good judgment of pathogenic bacteria,but they have a certain rate of misdiagnosis and missed diagnosis rate,and because of long time of report urine bacterial culture can＇t get timely and effective service to the clinical diagnosis and treatment. To understand its characteristics and the combined application can make up the deficiency of single index,so as to improve the diagnosis,treatment and curative effect of urinary tract infection.

关 键 词：尿液白细胞计数 细菌定量计数 细菌散点图 亚硝酸盐反应 尿液细菌培养 

分 类 号：R446.12[医药卫生—诊断学]