姜黄素对2型糖尿病肾病大鼠保护作用及机制研究  

Protective effect of curcumin on type 2 diabetic nephropathy rats and mechanism research

作　　者：王道周 裘志成[2] 杨艳[3] 张涤华[3] 

机构地区：[1]铜仁市人民医院肾脏内分泌血液科,铜仁554300 [2]遵义医学院附属医院肾病风湿科,遵义563003 [3]中山大学附属第一医院肾内科,广州510080

出　　处：《中国免疫学杂志》2017年第4期588-592,共5页Chinese Journal of Immunology

摘　　要：目的:探讨姜黄素对2型糖尿病肾病(DN)大鼠保护作用及可能的作用机制。方法:30只Wistar大鼠随机分为对照组、DN组和姜黄素组,每组10只。DN组和姜黄素组采用高脂饲料喂养后注射链脲佐菌素建立DN模型,造模成功后姜黄素组以200 mg/kg姜黄素灌胃,每天1次,对照组和DN组给予等量羧甲基纤维素钠灌胃,连续给药12周。末次给药后收集24 h尿量,称重,腹腔麻醉后心脏取血,检测血糖(Blood glucose,BG)、总胆固醇(Total cholesterol,TC)、甘油三酯(Triacylglyceride,TG)、血尿素氮(Blood urea nitrogen,BUN)、血肌酐(Serum creatinine,Scr)、尿微量白蛋白(Vrine microalbumin,m Alb),计算尿蛋白排泄率(Urinary protein excretion rate,UAER);处死大鼠,摘取双肾除去包膜后称重(KM),计算肾重指数(KI);多聚甲酚固定肾皮质用于苏木素-伊红(HE)染色,肾皮质制成匀浆检测MDA和SOD活性,Western blot检测肾组织中核因子相关因子2(Nrf2)和血红素加氧酶1(HO-1)蛋白表达。结果:DN组和姜黄素组BG、TC、TG、KI、BUN、Scr、UAER、MDA显著高于对照组,BM、KM、SOD显著低于对照组,且姜黄素组上述指标升高或降低幅度小于DN组,差异均有统计学意义(P<0.05);HE染色结果显示,与DN组比较,姜黄素组肾小管变性明显减轻,炎性细胞浸润明显减少;Western blot结果显示对照组Nrf2和HO-1蛋白表达显著低于DN组和姜黄素组,而姜黄素组显著高于DN组,差异均有统计学意义(P<0.05)。结论:姜黄素可能通过激活Nrf2/ARE信号通路,降低糖尿病肾病大鼠氧化应激、发挥肾脏保护作用。Objective： To explore the protective effect of curcumin on type 2 diabetic nephropathy rats and the possible mechanism. Methods： 30 Wistar rats were randomly divided into control group,the DN group and curcumin group,10 cases in each group,rats in DN and curcumin group were fed with high fat,after that they were used the streptozotocin injection to set up DN model. In addition,rats in curcumin group were given 200 mg/kg curcumin once a day,other two group were given sodium carboxymethylcellulose half-andhalf. After 12 weeks,the 24 h urine were collected at the last delivery,the blood glucose（ BG）,total cholesterol（ TC）,triglyceride（ TG）,blood urea nitrogen（ BUN）,serum creatinine（ Scr）,microalbu minuria（ m Alb） were tested,the urinary albumin excretion rate（ UAER） was calculated. Furthermore,rats were sacrificed,kidney mass weighed,and the kidney index（ KI） was calculated. Renal cortical was fixed with polymers cresol for hematoxylin-eosin（ HE） staining,renal cortical was also made renal cortex homogenate and the SOD activity,MDA were detected,the expression of nuclear factor related factor 2（ Nrf2） and heme oxygenase 1（ HO-1） protein in the kidney tissues was detected with Western blot detection. Results： BG,TC,TG,KI,BUN,Scr,UAER,MDA of DN group and curcumin group were significantly higher than control group,and BM,KM,SOD was significantly lower than the control group,the amplitude raising or reducing in curcumin group were less than DN group,the difference had statistical significance（ P0. 05）; HE staining results showed that,compared with DN group,renal tubule degeneration in curcumin group was obviously reduced,inflammatory cells infiltration was decreased significantly; Western blot results showed that expression of Nrf2 and HO-1 protein in control group were significantly lower than DN group and curcumin group,and curcumin group was significantly higher than the DN group,the differences were statistically significant（ P0. 05）. Conclusion： Curcumin m

关 键 词：姜黄素 2型糖尿病肾病 氧化应激 Nrf2/ARE信号通路 

分 类 号：R285.5[医药卫生—中药学]