EPO和HBSP在尿源性脓毒血症致兔急性肾损伤和炎症反应中的保护作用  

Evaluation of the protective effect of EPO and HBSP on kidney injury and inflammatory response induced by urinary-derived sepsis

作　　者：盛董董 乃比江.毛拉库尔班 阿斯木江.阿不拉 安尼瓦尔.牙生[1] 宋光鲁[1] SHENG Dongdong Naibijiang Maolakuerban Asimujiang Abula Anniwaer Yasheng SONG Guanglu(Department of Urology Center, the Frist Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, Chin)

机构地区：[1]新疆医科大学第一附属医院泌尿中心,乌鲁木齐830054

出　　处：《新疆医科大学学报》2017年第6期787-791,796,共6页Journal of Xinjiang Medical University

基　　金：新疆医科大学研究生创新创业项目(CXCY021)

摘　　要：目的评估促红细胞生成素(EPO)及其衍生肽(HBSP)在尿源性脓毒血症致兔急性肾损伤及炎症反应中的保护作用。方法将24只新西兰大白兔随机均分为US组[将800μg/kg内毒素(LPS)1mL注入左侧输尿管内,在注射点上方结扎输尿管,逐层缝合关闭腹腔]、Sham组(不结扎输尿管和注射LPS,其余操作同US组)、EPO治疗组(建模后经耳缘静脉给予EPO 5000IU/kg体质量)、HBSP治疗组(建模后经耳缘静脉给予HBSP60μg/kg体质量,每6小时1次)。采用肾盂高压法建立上尿路急性梗阻并脓毒血症新西兰大白兔模型。于术前及术后12、24、36h采血检测血肌酐(Cr)、尿素氮(BUN)、谷丙转氨酶(ALT)、谷草转氨酶(AST),酶联免疫吸附测定(ELISA)法检测血清中肿瘤坏死因子(TNF)-a、白细胞介素(IL)-6。术后36h行HE染色光学显微镜下观察肾脏、肝脏、肺脏组织病理学变化。US组结果与Sham组比较,确定尿源性脓毒血症兔模型是否建立。EPO治疗组、HBSP治疗组结果与US组比较,评估EPO和HBSP在尿源性脓毒血症致兔急性肾损伤及炎症发应中的保护作用。结果与Sham组相比,术后12、24、36h,US组Cr、BUN、ALT、AST、TNF-a、IL-6均明显升高,差异有统计学意义(P<0.01),与US组相比,EPO治疗组及HBSP治疗组各时间点Cr、BUN、ALT、AST、TNF-a、IL-6水平无明显下降,差异无统计学意义(P>0.05)。光镜下观察组织病理学改变,与US组相比,EPO治疗组及HBSP治疗组肾脏、肝脏及肺脏组织损伤评分差异无统计学意义(P>0.05)。结论促红细胞生成素及其衍生肽对尿源性脓毒血症兔急性肾损伤无明显保护作用,对炎症反应亦无明显抑制作用。Objective To evaluate the protective effect of erythropoietin （EPO） and Helix B surface peptide （HBSP） on kidney injury and inflammatory response induced by urinary-derived sepsis. Methods Twenty- four New Zealand rabbits were randomly divided into Sham group, US group （800 μg/kg LPS 1 mL into the left ureter）, EPO treatment group （EPO 5 000 IU/kg was given via the ear vein）, HBSP treatment group （HBSP 60 μg/kg was given by ear vein, once every six hours）. Upper urinary tract obstruction and acute infection was induced to establish sepsis model. Before surgery and 12 h, 24 h, 36 h after surgery, blood was collected to examine creatinine （Cr）, blood urea nitrogen （BUN）, alanine aminotransferase （ALT）, aspertate aminotransferase （AST）, tumor necrosis factor-a （TNF-a） and interleukin-6 （IL-6） are measured by Enzyme-linked immunosorbent assay （ELISA）. Kidney, liver and lung tissues were stained with hematoxylin and eosin （H＆E） 36 h after surgery, in addition, morphological changes were observed under the light microscope. The results of the US group were compared with the Sham group to explore whether the model of urinary sepsis rabbits was established. EPO treatment group and HBSP treatment group were compared with the US group to evaluate the protective effect of EPO and HBSP on acute renal injury and inflammatory response in urinary sepsis rabbits. Results At 12 h, 24 h and 36 h after surgery, levels of Cr, BUN, ALT, AST, TNF-a and IL-6 were all significantly elevated in US group compared with the Sham group （P 〈0.01）.When compared with US group, no significant decline was observed in EPO treatment group and HBSP treatment group （P 〉 0.05）. The histopathological changes were observed under light microscope. Compared with US group, there was no significant difference in the injury scores of kidney, liver and lung tissue in EPO treatment group and HBSP treatment group （P〉0.05）. Conclusion EPO and HBSP have no significant protective effect on

关 键 词：促红细胞生成素 衍生肽 尿源性脓毒血症 肾损伤 炎症反应 

分 类 号：R515.3[医药卫生—内科学]