油橄榄叶中活性成分的提取工艺考察  

Extraction Technology for Active Components in Olive Leaves

作　　者：王和宇[1,2] 李春娜[1,3] 秦玲玲[1] 张晶[2] 徐暾海[1,3] 刘铜华[1] WANG Heyu LI Chunna QIN Lingling ZHANG Jing XU Dunhai LIU Tonghua(Key Laboratory of TCM Health Education Ministry, Beijing University of Traditional Chinese Medicine, Beijing 100029, Chino Chinese Medicinal College, Jilin Agricultural University School of Traditional Chinese Materia Medico, Beijing University of Traditional Chinese Medicine)

机构地区：[1]北京中医药大学中医养生学教育部重点实验室,北京100029 [2]吉林农业大学中药材学院 [3]北京中医药大学中药学院

出　　处：《西部中医药》2017年第3期39-42,共4页Western Journal of Traditional Chinese Medicine

基　　金：国家国际科技合作专项项目(编号2012DFG31550);北京中医药大学创新团队项目(编号2011-CXTD-19)

摘　　要：目的:考察油橄榄(Olea europaea Linn)叶中活性成分的最佳提取工艺。方法:采用HPLC法测定油橄榄叶中木犀草苷、橄榄苦苷,并使用超声波提取法进行提取,考察料液比,溶剂分数,提取时间,超声功率对含量的影响。在单因素基础上,结合正交试验考察溶剂分数、料液比、提取时间对橄榄苦苷含量的影响,确定最佳提取工艺。结果:油橄榄叶中活性成分的最佳提取工艺为80%甲醇溶液30 mL,超声400W提取30分钟,提取次数1次。结论:采用该方法提取油橄榄叶中活性成分简单易行。Objective：To study the best extraction technology for active components in olive leaves. Methods：Galuteolin, oleuropein in olive leaves were determined by HPLC method, and extracted by ultrasonic method. Ef-fects of the solid-liquid ratio, solvent fraction, extraction time and ultrasonic power on contents were inspected. Based on single factor tests and combined with orthogonal test, effects of the solvent fraction, solid-liquid ratio and extraction time on the content of oleuropein were inspected to determine the best extraction technology. Results：The best extraction technology for active components in olive leaves was extracting for 30min with 30mL 80%methanol solution under the condition of 400W ultrasound, and the extraction time was once. Conclusion：This method is sim-ple and practicable for extracting the active components in olive leaves.

关 键 词：油橄榄叶 HPLC 木犀草苷 橄榄苦苷 超声波提取法 正交试验 提取工艺考察 

分 类 号：R284[医药卫生—中药学]