Fast Mineralization of Densely Packed Hydroxyapatite Layers in the Presence of Over-expressed Recombinant Amelogenin  

作　　者：王梦湖 XIE Hao XIE Jingjing PING Hang TAN Tiening JI Wei 傅正义 

机构地区：[1]State Key Laboratory of Advanced Technology for Materials Synthesis and Processing,Wuhan University of Technology [2]School of Science,Wuhan University of Technology

出　　处：《Journal of Wuhan University of Technology(Materials Science)》2017年第2期256-263,共8页武汉理工大学学报（材料科学英文版）

基　　金：Funded by the National Natural Science Foundation of China(51521001);the Ministry of Science and Technology of China(2015DFR50650);the Fundamental Research Funds for the Central University(WUT 2016IB006)

摘　　要：Learning from the process of biominerals formation provides tremendous ideas for developing advanced synthesis techniques. According to the structure formation of tooth enamel, a recombinant amelogenin his-Amel X was designed and constructed. The protein was over-expressed and could be conveniently purified in one-step heat treatment. The mineralization process of hydroxyapatite was initiated by enzyme AP and regulated by the recombinant amelogenin. Effects of solution pH value and mineralization duration were studied. It was demonstrated that his-Amel X could induce the nucleation of apatite and quicken the growth rate at pH 7.0-7.4, while impeded hydroxyapatite growth at pH 6.8. Moreover, a much denser layer of hydroxyapatite was achieved with the addition of his-Amel X. The present study may not only provide insight into the formation of natural biomaterials but also open a new path to prepare materials under environmentally benign conditions.Learning from the process of biominerals formation provides tremendous ideas for developing advanced synthesis techniques. According to the structure formation of tooth enamel, a recombinant amelogenin his-Amel X was designed and constructed. The protein was over-expressed and could be conveniently purified in one-step heat treatment. The mineralization process of hydroxyapatite was initiated by enzyme AP and regulated by the recombinant amelogenin. Effects of solution pH value and mineralization duration were studied. It was demonstrated that his-Amel X could induce the nucleation of apatite and quicken the growth rate at pH 7.0-7.4, while impeded hydroxyapatite growth at pH 6.8. Moreover, a much denser layer of hydroxyapatite was achieved with the addition of his-Amel X. The present study may not only provide insight into the formation of natural biomaterials but also open a new path to prepare materials under environmentally benign conditions.

关 键 词：biomineralization recombinant amelogenin hydroxyapatite bioprocess inspired synthesis 

分 类 号：R318.08[医药卫生—生物医学工程]