植物凝集素上调小鼠免疫器官MDSC细胞的研究  

Study on the effect of phytohaemagglutinin on upregulating MDSCs of immune organ in mice

作　　者：张潇涵[1] 李江超[1] 刘擎[1] 周泽启 仰明明 王丽京[1] 

机构地区：[1]广东药科大学血管生物学研究所,广东广州510006

出　　处：《转化医学电子杂志》2017年第4期15-18,共4页E-Journal of Translational Medicine

基　　金：国家自然科学基金(81472336;31471290);广东省科技计划(51343072;2014A020212313;2015A030302086)

摘　　要：目的:探讨小鼠腹腔注射植物凝集素(PHA)后血液、脾脏、骨髓中的MDSC数量的变化,明确PHA对小鼠血液、脾脏、骨髓MDSC的作用.方法:小鼠腹腔注射PHA后,提取小鼠血液、脾脏及骨髓进行细胞裂解,标记抗体后采用流式细胞仪进行检测和分析.结果:PHA处理组小鼠的骨髓、脾脏、外周血中MDSC百分比明显比生理盐水组高,骨髓中生理盐水组双阳性平均为36.6%,而PHA组双阳性达到56.4%,差异有统计学意义(P<0.05).脾脏中,生理盐水组双阳性平均为3.5%,然而植物凝集素组双阳性能达到11.5%左右,差异有统计学意义(P<0.05).外周血中,PHA处理组MDSC百分比明显升高,对照组MDSC平均百分比占4.6%,而PHA组MDSC平均百分比占19.8%,差异有统计学差异(P<0.05).结论:植物凝集素腹腔刺激小鼠后,可促使小鼠血液、脾脏、骨髓中MDSC浓度的上调.ADd： To investigate the changes in the number of MDSCs in blood, spleen and bone marrow after intraperitoneal injection of phytohaemagglutinin （PHA） in mice, and confirm the effects of PHA on MDSC in blood, spleen and bone marrow. METHODS ： After intraperitoneal injection of PHA in mice, the red cell extracted from the blood, spleen and bone marrow was ly- sised, and MDSCs altering of labeled antibody was detected and analysed using flow cytometry. RESULTS： Percentage of MDSCs treated by Phytohacmagglutinin was significantly higher than that of the control group in bone marrow, spleen, peripheral blood. More detail, MDSCs in control group was 36.6% in bone marrow, while MDSCs in Phytohaemagglutinin group reached 56.4%, and the difference was statistically significant （P〈0.05）. Average val- ue of MDSCs in spleen was 3.5% in control group, however that of the Phytohaemagglutinin group was 11.5%, and the difference was statistically significant （P〈0.05）. Similarly, it is difference in peripheral blood （4.6% vs 19.8%）. CONCLUSION： PHA can promote up-regulation of MDSC in blood, spleen and bone marrow in mice.

关 键 词：植物凝集素 MDSC细胞 细胞分化 

分 类 号：Q95-33[生物学—动物学]