HPLC法测定纤维低聚糖的研究  

The HPLC Determination of Cello-oligosaccharides

作　　者：宋维春 裴志胜 孙宏元 梁志鹏 李欣怡 徐云升 

机构地区：[1]海南热带海洋学院生命科学与生态学院,海南三亚572022

出　　处：《海南热带海洋学院学报》2017年第2期70-73,共4页Journal of Hainan Tropical Ocean University

基　　金：国家自然科学基金项目(21262026)

摘　　要：本研究运用HPLC法,采用Inertsil NH2柱、示差折光检测器,以乙腈-水为流动相,探索纤维二糖、纤维三糖、纤维四糖、纤维五糖的检测条件.实验结果表明:在检测器灵敏度为+8,流速为1 ml/min,进样量为10μL条件下,适宜检测条件是:流动相为乙腈:水(v/v)=65:35,色谱柱温度为41℃.在该检测条件下,峰高Y与浓度X的定量检测方程为:纤维二糖Y=3001X-147.6;纤维三糖Y=915.9X+411.6;纤维四糖Y=1328X+10002;纤维五糖Y=2029X-1357.In this paper, the HPLC - RID determination conditions of cellobiose, cellotriose, cellotetraose and cellopentaose were explored with Inertsil NH2 chromatographic column. The results showed that the appropriate HPLC conditions on the determination of cello - oligosaccharides are that the mobile phase is acetonitrile/wa- ter （v/v） = 65/35; the velocity of mobile phase, lml/min; column temperature, 41℃ ; and the sensitivity of detector, ＋ 8. Correlations of peak height（Y） and concentration（X） are Y = 3001X - 147.6 for cellobiose, Y = 915.9X ＋ 411.6 for cellotriose, Y = 1328 X ＋ 10002 for cellotetraose, and Y = 2029X - 1357 for cellopentaose.

关 键 词：HPLC法 纤维二糖 纤维三糖 纤维四糖 纤维五糖 

分 类 号：O629.1[理学—有机化学] TS20[理学—化学]