液相色谱-串联质谱法测定大鼠体内凤仙萜四醇苷B血药浓度及其药动学研究  

Study on Plasma Concentration and Pharmacokinetics of Hosenkoside B in Rats by LC-MS/MS

作　　者：滕学鹏[1,2,3,4] 邓志鹏[2,3,4] 董伟[5] 邹本奎[5] 仲浩[2,3,4] 

机构地区：[1]济南大学山东省医学科学院医学与生命科学学院,山东济南250200 [2]山东省医学科学院药物研究所,山东济南250062 [3]国家卫生部生物技术药物重点实验室,山东济南250062 [4]山东省罕少见病重点实验室,山东济南250062 [5]山东省肿瘤防治研究院,山东济南250117

出　　处：《食品与药品》2017年第2期88-91,共4页Food and Drug

基　　金：山东省自然科学基金-三院联合基金项目(编号:ZR2015YL048)

摘　　要：目的建立液相色谱-串联质谱法(LC-MS/MS)测定大鼠血浆样品中凤仙萜四醇苷B浓度的方法,并进行药动学研究。方法取6只大鼠,尾静脉单剂量注射凤仙萜四醇苷B 1.0 mg/kg,分别于给药前和给药后2,5,20,40,60,90,120,180 min眼眶隐静脉丛采血,采用LC-MS/MS测定其血药浓度,并采用DAS计算药动学参数。结果凤仙萜四醇苷B在大鼠体内的主要药动学参数t_(1/2z)为0.518±0.114 h,C_(2min)为159.11±9.17 ng/m L,AUC0-t为142.86±23.53 ng·h/m L。结论本方法选择性好、灵敏度好、准确度高,可为下一步该化合物临床前药动学研究提供方法学依据。Objective To establish a sensitive method for determination of concentrations of hosenkoside B in rat plasma and to study its pharmacokinetics by LC-MS/MS. Methods 6 rats were given single dose of 1.0 mg/kg hosenkoside B via tail vein injection. Blood samples were harvested from orbital saphenous venous plexus before administration and 2, 5, 20, 40, 60, 90, 120, 180 min after dosing. The plasma concentrations of hosenkoside B were determined by LC-MS/MS and the pharmacokinetic parameters were calculated by DAS software. Results Main pharmacokinetic parameters were as follows： t1/2z, 0.518±0.114 h; C2min, 159.11±9.17 ng/mL; AUC0-t, 142.86±23.53 ng？h/mL. Conclusion The method is specific, sensitive, and accurate. It can provide methodological evidence for further preclinical pharmacokinetic study of hosenkoside B.

关 键 词：凤仙萜四醇苷B 液相色谱-串联质谱法 大鼠血浆 药动学 

分 类 号：R969.1[医药卫生—药理学]