响应面法优化斑花黄堇中总生物碱的提取工艺  

Optimization of Ultrasonic Extraction of Total Alkaloids from Corydalis Conspersa Maxim

作　　者：确生[1,2] 王志波[2] 李成思[2] 林鹏程[2] 陈卫东[2] 

机构地区：[1]天津大学管理与经济学部,天津300072 [2]青海民族大学药学院/青海省青藏高原植物化学重点实验室,西宁810007

出　　处：《中国现代应用药学》2017年第3期358-362,共5页Chinese Journal of Modern Applied Pharmacy

基　　金：青海省自然科学基金项目(2016-ZJ-903)

摘　　要：目的优化盐酸回流提取藏药斑花黄堇中总生物碱的提取工艺。方法根据单因素试验结果,选择影响总生物碱提取效果较大的盐酸浓度、液料比、提取温度为自变量,以总生物碱的得率为响应值,进行Box-Benhken中心组合试验设计,采用响应面法评估这些因素对总生物碱提取效果的影响。结果提取斑花黄堇中总生物碱的最佳工艺条件为液料比35 m L·g^(-1),盐酸浓度为0.4%,提取温度为75℃,得斑花黄堇中总生物碱的提取率为0.46 mg·g^(-1)。结论该法优化的提取工艺科学可靠,可用于斑花黄堇总生物碱的提取生产。OBJECTIVE To optimize ultrasonic-assisted extraction process of total alkaloids from Corydalis conspersa Maxim. METHODS The hydrochloric acid concentration, liquid-feed ratio and extraction temperature, which were major factors influencing the extraction according to the single factor experiments, were selected as independent variables, and through using the extraction rate of total alkaloids as response values and making the Box-Benhken central combinational design, the effects of the above factors on the extraction were estimated by response surface methodology. RESULTS The results indicated that the optimal extraction conditions were as follows： 35 m L·g^-1 of liquid-feed ratio, 0.4% of hydrochloric acid concentration, and 75 ℃ extraction temperature. Under these conditions, the content of total alkaloids was 0.46 mg·g^-1 from Corydalis conspersa Maxim by ultraviolet spectrophotometer. CONCLUSION The optimized parameters from the expriments are accurate, which can be used for the extraction and production of total alkaloids from Corydalis conspersa Maxim.

关 键 词：斑花黄堇 生物碱 提取工艺 响应面法 

分 类 号：R284.1[医药卫生—中药学]