大鼠溺死后肺组织与血清IL-1β、IL-13mRNA表达变化  

The changes on expression of IL-1β and IL-13 mRNA in lung tissue and serum after drowning rats

作　　者：姜美玲[1] 郭文平 赵晋芳[3] 芦俊峰 梅泽 刘佳佳[1] 梁新华[1] 

机构地区：[1]山西医科大学法医学院,山西太原030001 [2]晋中市公安局城区分局,山西晋中030600 [3]山西医科大学公共卫生学院,山西太原030001

出　　处：《中国法医学杂志》2017年第2期137-140,共4页Chinese Journal of Forensic Medicine

基　　金：山西省自然科学基金资助项目(2011011041-3)

摘　　要：目的检测大鼠肺组织和血清中IL-1β、1L-13mRNA表达变化,探讨IL-1β、IL-13mRNA的变化情况与溺死的关系。方法 12只SD大鼠随机分为对照组、溺死组(实验组)。分别提取各组大鼠右侧肺下叶及右心室血清,应用TaqMan探针法检测肺组织及右心室血清IL-1β、IL-13mRNA的表达情况。结果 (1)溺死组大鼠肉眼观察:体表征象及解剖所见符合生前溺死特征。(2)肺组织中IL-1β、IL-13mRNA表达检测:与对照组比较,实验组IL-1β、IL-13表达呈下降趋势,差异无统计学意义(p>0.05)。(3)血清中IL-1β、IL-13mRNA表达检测:与对照组比较,实验组IL-1β表达显著升高(p<0.01)、IL-13表达显著升高(p<0.01)。结论 (1)IL-1β、IL-13 mRNA在溺死组肺组织中表达呈下降趋势,可能是大鼠溺死出现代偿性抗炎反应综合症导致免疫无能的表现。(2)IL-1β、IL-13 mRNA在血清中表达明显升高,可能与溺水应激及溺死后急性肺损伤创伤应激有关。Objective To observe the change in IL-1β, IL-13mRNA expression in drowning rat lungsand serum, so as to investigate the significance of IL-1βand IL-13 mechanism in the development of drowning.Methods SD rats were randomly divided into control group, drowning group.Then using TaqMan probe method todetermine the expression ofIL-1β and IL-13 mRNA in Right lower lobe of lung tissue and the serum of right ventricle,which were extracted respectively from each group of rats. Results （1） The lung tissue morphological changesTypical appearance signs and anatomy of drowning group meet ante-mortem drowning feature. （2） The expressionofIL-1β, IL-13 in lung tissue： compared with the control group, the expression of IL-1β and IL-13 were slightlydecreased, which has no statistical significance. （3） The expression of IL-1β and IL-13 in serum： compared with thecontrol group, the expression of IL-1β and IL-13 were significant increased, both of which has statistical significance.Conclusion （1）The expression of IL-1βand IL-13 were decreased in lung tissue may be due to drowned rats presentcompensatory anti-inflammatory response syndrome which causes immune incompetent performance. （2） The expressionof IL-1β and IL-13 were significant increased in serum may be relate to drown stress and drowning associated acutelung injury after traumatic stress.

关 键 词：法医病理学 溺死 TaqMan探针法 细胞因子IL-1Β IL-13 

分 类 号：D919[医药卫生—法医学]