β-catenin在兔颈总动脉套管导致的动脉粥样硬化血管壁中高表达  

β-catenin high expression in atherosclerotic vessel wall caused by rabbit carotid artery cannula

作　　者：刘月华[1] 生燕 涂应杰 杨兴[1] 欧刚卫[1] 生欣[1] 

机构地区：[1]遵义医学院基础医学院生物化学教研室,贵州遵义563000 [2]遵义医学院基础医学院形态学实验室,贵州遵义563000

出　　处：《重庆医学》2017年第12期1588-1591,共4页Chongqing medicine

基　　金：国家自然科学基金资助项目(31360278);贵州省科技厅联合基金项目[黔科合J字LKZ(2013)28号];遵义医学院博士启动基金(F566)

摘　　要：目的探讨兔颈总动脉套管对动脉粥样硬化形成及β-catenin表达的影响。方法将15只体质量为(2.0±0.2)kg的2个月龄健康新西兰兔分为3组,分别为高脂饲养组、左侧颈总动脉套管组和左侧颈总动脉套管+高脂饲养组,每组5只,取左侧颈总动脉套管组右侧血管为对照组。饲养9周后处死动物,取各组兔颈总动脉血管;实时荧光定量PCR(RT-PCR)及免疫组织化学检测兔颈总动脉血管组织中β-catenin mRNA和蛋白表达定位。结果RT-PCR结果显示左侧颈总动脉套管组β-catenin mRNA表达较对照组、高脂饲养组、左侧颈总动脉套管+高脂饲养组上调。免疫组化结果显示,除对照组外,各组胞质中均有β-catenin蛋白定位,且左侧颈总动脉套管组、左侧颈总动脉套管+高脂饲养组中主要定位于内膜增生处。结论β-catenin在兔颈总动脉套管导致的动脉粥样硬化血管壁中高表达。Objective To investigate the effects of rabbit carotid artery cannula on atherosclerosis formation andβ-catenin expression.Methods Fifteen 2-month-old New Zealand rabbits weighing（2.0±0.2）kg were randomly divided into three groups,high-fat diet group,left common carotid artery cannula group and left common carotid artery cannula plus high-fat diet group,5cases in each group,and taking the right blood vessels in the left common carotid artery cannula group served as the control group.The animals were sacrificed after 9-week feeding,and the total rabbit carotid artery in each group was taken;the real-time PCR and immunohistochemistry were used to detectβ-catenin mRNA and protein expression targeting in rabbit common carotid artery tissue.Results The real-time PCR results showed that theβ-catenin mRNA expression in the left common carotid artery cannula group was higher than that in the control group,high fat diet group and the left common carotid artery cannula ＋ high-fat diet group.The immunohistochemistry results showed that,except for the control group,various groups had theβ-catenin protein location in the cytoplasma,moreover which in the left carotid artery cannula group and left common carotid artery cannula＋high-fat diet group mainly located in the area of intimal hyperplasia.Conclusion β-catenin is highly expressed in the atherosclerotic vessel wall caused by rabbit carotid artery cannula.

关 键 词：颈总动脉套管 动脉粥样硬化 Β-CATENIN 动物实验 兔 

分 类 号：R543.5[医药卫生—心血管疾病]