大孔树脂分离纯化维药洋甘菊中黄酮工艺研究  

The optimal purification technology of flavone in Uygur medicine Matricaria chamomilla L. by macroporous resin

作　　者：兰卫[1,2] 郭玉婷[3] 胡江兰[4] 安冬青[1] 倪健[2] 

机构地区：[1]新疆医科大学中医学院,新疆乌鲁木齐830011 [2]北京中医药大学中药学院,北京100029 [3]新疆医科大学对口协作办公室,新疆乌鲁木齐830011 [4]新疆农业大学食品科学与药学学院,新疆乌鲁木齐830052

出　　处：《时珍国医国药》2017年第3期575-578,共4页Lishizhen Medicine and Materia Medica Research

基　　金：新疆维吾尔自治区科技支撑计划项目(No.201133128)

摘　　要：目的筛选大孔树脂分离纯化洋甘菊黄酮的最佳工艺。方法以黄酮的吸附量为指标,采用单因素试验法对影响洋甘菊提取物纯化效率的因素进行优化,采用UV测定纯化后固形物中总黄酮的浓度。结果最佳洗脱程序为,选定D101大孔树脂,上样浓度为0.8269 mg·ml^(-1),最大上样量为16 BV(16倍柱体积),上样速度为0.081 BV·min^(-1),依次用6BV的蒸馏水以0.081 BV·min^(-1)的流速洗脱,弃去洗脱液,再用6 BV的70%乙醇以0.041 BV·min^(-1)的流速洗脱,收集洗脱液。纯化得到的固形物中洋甘菊黄酮含量为51.37%,总黄酮的转移率为80.94%。结论纯化工艺简便、稳定,能提高洋甘菊中活性组分的含量。Objective To select the optimal purification technology of flavone in Matricaria chamomilla L. with macroporous resin. Methods Mono factor test were employed to investigate the main parameters which may affect the purification procedures with the concentration of flavone as index. The contents of the total flavone were verified by UV in purified dried solid. Results The optimal procedure was using D101 macroporous resin, the content of flavone was 0. 8269 mg · ml-1 , the volume was 16 BV, the flow rate was 0. 081 BV ·min-1. To elute with distilled water, the volume was 6 BV, the flow rate was 0. 081 BV · min-1, giv- ing up the eluate. To elute with 70% ethanol, the volume was 6 BV, the flow rate was 0. 041 BV· min-1, gathering the eluate. The content of flavone reached 51.37%, the transfer rates of flavone reached 80.94%. Conclusion The optimized technology is simple, stable, can significantly improve the contents of active constituents in Matricaria chamomilla L.

关 键 词：洋甘菊 大孔树脂 纯化 黄酮 

分 类 号：R284.2[医药卫生—中药学] R291.5[医药卫生—中医学]