蜂胶化学成分的LC-IT-TOF-MS鉴别  

Identification of major constituents from propolis by LC-IT-TOF-MS

作　　者：姚静[1] 孙建国[2] 吴亮[2] 汪豪[1] 王广基[2] 叶文才[3] 

机构地区：[1]中国药科大学天然药物化学教研室,南京210009 [2]中国药科大学药物科学研究院,南京210009 [3]暨南大学药学院中药及天然药物研究所,广州510632

出　　处：《中国药科大学学报》2017年第2期178-183,共6页Journal of China Pharmaceutical University

基　　金：国家自然科学基金资助项目(No.81573309)~~

摘　　要：建立液相色谱串联离子阱飞行时间质谱法(LC-IT-TOF-MS)鉴定中国蜂胶的化学成分。采用Shim-pack VP-ODS色谱柱(2.0 mm×150 mm,5μm),流动相为乙腈-0.2%甲酸水溶液,梯度洗脱,流速0.3 mL/min,采用ESI-IT-TOF检测,负离子模式扫描。根据色谱峰精确相对分子质量、碎片离子信息、及液相色谱保留时间比较鉴别化合物。采用LC-IT-TOF-MS法鉴定了中国蜂胶(山东泰安)中31个成分,包括25个黄酮类化合物和6个酚酸类化合物。结果表明,采用LC-IT-TOF-MS方法可以快速、准确地对蜂胶中各成分进行有效的定性分析。A qualitative analytical method of liquid chromatography coupled with ion trap time-of-flight mass spectrometry（LC-IT-TOF/MS）was developed for identification of major constituents in propolis from China（Shandong）. The LC-IT-TOF-MS was performed on a Shim-pack VP-ODS column（2. 0 mm×150 mm, 5 μm）with the mobile phase consisting of acetonitrile and water containing 0. 2% formic acid in gradient mode, and the flow rate was set at 0. 3 mL/min. Negative ion mode was used for IT-TOF-MS. According to the accurate molecular weight, MS fragment pathway, comparison with the retention time of reference compounds, total 31 compounds, including twenty-five phenolic acids and six flavonoids were identified. The LC-IT-TOF-MS qualitative analysis method can be used for analyzing major components of propolis quickly and accurately.

关 键 词：蜂胶 LC-IT-TOF-MS 定性分析 

分 类 号：R284[医药卫生—中药学]