IRAK-M mRNA在急性脑出血大鼠肝Kupffer细胞中的表达及涤痰通瘀方的干预作用  

Expression of IRAK-M mRNA in Kupffer cells of liver during acute cerebral hemorrhage and effects of Ditantongyu decoction

作　　者：李蕊[1] 赵海滨[1] 王昀[1] 吴羿姗 张晗[1] LI Rui ZHAO Haibin WANG Yun WU Yishan ZHANG Han(The Third Affiliated Hospital of Beijing University of Chinese Medicine, Beijing 100029, China)

机构地区：[1]北京中医药大学第三附属医院,北京100029

出　　处：《吉林中医药》2017年第4期389-393,共5页Jilin Journal of Chinese Medicine

基　　金：国家自然科学基金(No.81373590)

摘　　要：目的研究急性脑出血大鼠肝Kupffer细胞(KCs)中白细胞介素-1受体相关激酶-M(IRAK-M)的表达变化及涤痰通瘀方对急性脑出血大鼠肝损害的保护作用。方法将健康SD大鼠随机分为正常组、急性脑出血模型组和涤痰通瘀方组(酒大黄、胆南星、石菖蒲、蒲黄、三七、水蛭),尾状核注射Ⅶ型胶原酶法建立大鼠急性脑出血模型,按10 mL/kg灌胃,正常组和模型组给予等量生理盐水灌胃,上、下午各1次,连续4 d,第4天末次给药40 min后腹主动脉取血,分离血清,-20℃冻存备用。密度梯度离心法提取正常大鼠肝KCs,分别给药体外培养6、12、24 h后收集标本,实时荧光定量(PCR)检测各时相点IRAK-4 mRNA表达。结果脑出血模型组在各时间点IRAK-M mRNA表达水平[(0.749±0.045)、(0.641±0.054)、(0.728±0.056)]较正常组[(1.108±0.066)、(0.865±0.038)、(0.758±0.041)]均明显降低(P<0.05);中药治疗组的IRAK-M mRNA表达水平[(0.902±0.062)、(0.780±0.033)、(0.896±0.046)]较脑出血模型组明显升高(P<0.05)。结论涤痰通瘀方可以提高KCs内IRAK-M mRNA的表达,对急性脑出血应激性肝损害具有一定的保护作用。Objective To investigate the change of expression of IRAK-M mRNA in Kupffer cells （KCs） caused by hepatic stress injury in rats with acute cerebral hemorrhage and protective effect of Ditantongyu decoction on hepatic injury. Methods Healthy SD rats were randomly divided into three groups： blank control group, cerebral hemorrhage group and Ditantongyu decoction group （Jiudahuang, Dannanxing, Shichangpu, Puhuang, Sanqi, Shuizhi）. Cerebral hemorrhage was induced by injecting collagenase Ⅶ into the caudate nucleus of rats. Rats were given this preparation according to 10 mL/kg body weight, the rest of the group were received equivalent normal saline two times daily for four days, four days administration 40 min after treatment, serum was obtained from abdominal aorta and frozen spare parts -20 ℃ KCs were obtained through density gradient centrifugation and collected at 6, 12, and 24 h after drug administration. Expression of IRAK-4M rnRNA in KCs tested by using polymerase chain reaction （PCR） method. Results Compared with the blank control group, the expression levels oflRAK-M mRNA [ （ 0.749±0.045 ） , （ 0.641±0.054 ） , （ 0.728±0.056 ） ] in model group was reduced （P〈0.05）. The expression levels of IRAK-MmRNA [ （ 1.108±0.066 ） , （0.865±0.038） , （0.758±0.041） ] of Chinese medicine group elevated significantly compared with model group（P〈0.05）, but it decreased in blank control group. Conclusion Ditantongyu decoction could increase the expression of IRAK-M mRNA in KCs, which is an important way for preventing and treating hepatic stress injury caused by acute cerebral hemorrhage.

关 键 词：白细胞介素-1受体相关激酶-M KUPFFER细胞 急性脑出血 应激性肝损害 涤痰通瘀 

分 类 号：R285.5[医药卫生—中药学]