金黄色葡萄球菌α-溶血素突变体H35A抑制α-溶血素的细胞毒性  

Staphylococcus aureus α-hemolysin mutant H35A inhibit cytotoxicity of wild α-hemolysin

作　　者：孙倩男 郑峰[2] 周婷婷[2] 岳岩[3] 王怡雯[2] 朱旭辉[2] 王长军[2] 余伯阳[1] 朱进[2] Sun Qiannan Zheng Feng Zhou Tingting Yue Yan Wang Yiwen Zhu Xuhui Wang Changjun Yu Boyang Zhu Jin(School of Traditional Chinese Pharmacology,Chinese Pharmaceutical University ,Nanjing 211198 Huadong Medical Institute of Biotechniques,Nanjing 210002 Department of Infectious Disease, Chinese PLA General Hospital ,Beijing 100853, China)

机构地区：[1]中国药科大学中药学院,江苏南京211198 [2]南京军区军事医学研究所,江苏南京210002 [3]中国人民解放军总医院传染科,北京100853

出　　处：《南京医科大学学报（自然科学版）》2017年第3期293-297,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：全军"十三五"医学科技重点项目(BWS14J046);全军青年培育项目(14QNP041)

摘　　要：目的:研究金黄色葡萄球菌α-溶血素(α-hemolysin,Hla)突变体H35A在人外周血单个核细胞(human peripheral blood mononuclear cells,h PBMC)和THP-1巨噬细胞中对于野生型Hla毒性作用的影响。方法:H35A与Hla共同作用于h PBMC和THP-1巨噬细胞,实时荧光定量PCR检测细胞中白介素(interleukin,IL)-1β、IL-6、肿瘤坏死因子(tumor necrosis factor,TNF)-α等炎症因子的m RNA转录水平差异,台盼蓝染色计算THP-1巨噬细胞存活率。结果:预孵育突变体H35A 1 h的h PBMC和THP-1巨噬细胞,经Hla刺激产生IL-1、IL-6、TNF-α的m RNA转录水平显著降低;台盼蓝染色计数发现,H35A与Hla共同作用的THP-1巨噬细胞存活率显著高于Hla单独处理组。结论:突变体H35A可抑制野生型Hla对于h PBMC和巨噬细胞的毒性作用,为金黄色葡萄球菌早期感染防治药物的研发提供了更多选择。Objective：To study the effect of Staphylococcus aureus α-hemolysin mutant H35 A on the wild type α-hemolysin（Hla）toxicity in human periphery blood mononuclear cells（h PBMC） and THP-1 macrophages. Methods：h PBMC and THP-1 macrophages were stimulated with both H35 A and Hla. The transcriptions of interleukin-1β（IL-1β）,interleukin-6（IL-6）,tumor necrosis factor-α（TNF-α） m RNA in h PBMC and THP-1 macrophages were assayed by real-time Q-PCR. The survival percentage of THP-1macrophages treated with Hla and H35 A was calculated with Trypan blue staining. Results：The transcriptions of IL-1,IL-6 and TNF-α m RNA in h PBMC and THP-1 macrophages treated with H35 A mutant were significantly decreased upon stimulation with Hla. The survival percentage of THP-1 macrophages treated with Hla and H35 A was significantly increased. Conclusion：H35A mutant can inhibit the toxic effect of Hla on human monocytes and macrophages,and it is expected to be a therapeutic drug used in the early stage of Staphylococcus aureus infection.

关 键 词：α-溶血素 H35A突变体 炎症因子 细胞毒性 

分 类 号：R378.11[医药卫生—病原生物学]