茵陈素对肺癌细胞增殖和细胞周期的影响  

The Effects of Capillarin On Proliferation and Cell Cycle of Lung Cancer Cell

作　　者：蒋幼凡[1] 沈庆[1] 白冰 

机构地区：[1]重庆医科大学附属二院肺科,重庆400010 [2]山东省医药工业研究所,山东济南250100

出　　处：《中国药业》2002年第8期30-31,共2页China Pharmaceuticals

摘　　要：目的:探讨茵陈素（6,7-二甲基香豆素）对肺癌细胞增殖和细胞周期的影响。方法:应用光镜、四氮甲唑蓝（MTT）方法和流式细胞术分析茵陈素对肺癌细胞形态学、生长和细胞周期的影响。结果:茵陈素能抑制肺癌细胞的增殖,并呈剂量依赖性,以160μg/mL抑制作用最明显,抑制率达52.4％。细胞被阻滞于G0/G1期,不能进入S及G2/M期,增殖指数明显下降。结论:茵陈素在体外对肺癌细胞具有抑制作用,通过抑制DNA合成,将细胞阻滞于G0/G1期,从而抑制细胞增殖。Objective: Discuss the effects of capillarin(6,7- dimethylcoumarin) on proliferation and cell cycle of Lung cancer cell. Methods: Apply microscope, MTT method and flow cytometry, analyse the effects of capillarin on morphology, grow and cell cycle of lung cancer cell. Results: Capillarin can dose dependently inhibit the proliferation of lung cancer cell, with the best effect of 160μg/mL which suppressing rate is up to 52.4%. The cell is blocked in G0/G1 phase, cannot enter S and G2/M phase, proliferation index obviously drops. Conclusion: Capillarin has inhibitory effect on lung cancer cell in vitro. Through suppressing DNA synthesis, it blocks the cell in the G0/G1 phase, thus suppress cell' s proliferationl.

关 键 词：茵陈素 细胞增殖 6 7-二甲基香豆素 肺癌 细胞周期 

分 类 号：R273.42[医药卫生—中西医结合] R285[医药卫生—中医肿瘤科]