5-氮杂-2'-脱氧胞苷预处理致敏百草枯对V79细胞的毒性观察  

Toxic Effect of Paraquat on Sensitized V79 Cells Pretreated with 5-aza-2'-deoxycytidine

作　　者：曲艳[1] 王晔[1] 陈美琼[1] 李祎[1] QU Yan WANG Ye CHEN Mei-qiong LI Yi(Dept.of Emergency, Affiliated Yan~an Hospital of Kunming Medical University, Kunming Yunnan 650051, China)

机构地区：[1]昆明医科大学附属延安医院急诊医学科,云南昆明650051

出　　处：《昆明医科大学学报》2016年第9期36-39,共4页Journal of Kunming Medical University

基　　金：云南省科技厅昆明医科大学应用基础研究;联合专项基金资助项目(2013FB190)

摘　　要：目的观察DNA甲基化调控对预处理致敏百草枯V79细胞的影响.方法采用DNA甲基化特异性抑制剂5-氮杂-2'-脱氧胞苷(5-Aza-2'-dc)对V79细胞进行预处理12 h,然后加入百草枯作用12 h,采用显微镜观察V79细胞形态结构,应用MTT法、台盼蓝染色法计数检测各实验组中细胞存活状况.结果显微镜检查观察到5-Aza-2'-dc与百草枯联合作用组比5-Aza-2'-dc组、百草枯组V79细胞贴壁能力减弱、细胞体积变小,噻唑蓝(MTT)比色法检测到联合作用组细胞存活率(54.47±3.04)%显著低于5-Aza-2'-dc组(95.52±0.90)%、百草枯组(89.68±4.26)%(P<0.05),台盼蓝染色计数算出联合作用组死细胞率(53.58±1.57)%显著高于5-Aza-2'-dc组(7.44±2.31)%、百草枯组(12.90±1.21)%(P<0.05).结论 5-Aza-2'-dc能够促进百草枯对V79细胞的损伤.Objective To study the effect of DNA methylation regulation on the toxic effect of paraquat on the sensitized V79 cells t pretreated with 5-aza-2＇-deoxycytidine. Methods V79 cells were treated by5-aza-2＇-deoxycytidine（5-Aza-2＇-dc） for 12 h, which is a DNA methylation inhibitor,and then treated with paraquat for 12 h. The morphological changes of V79 cells were observed by microscopy and the cell viability was determined by MTT assay and trypan blue staining method.Results Microscopic examination showed that the combination of 5-Aza-2＇-dc and paraquat had stronger effect in inhibiting the growth of V79 cells（the cells became smaller and poorer adhensive ability） than single 5-Aza-2＇-dc or paraquat. MTT assay showed that cell viability in the combination group（54.47±3.04） % was significantly lower than the 5-Aza-2＇-dc group（95.52 ± 0.90） %and paraquat group（89.68±4.26） %（P0.05）. Trypan blue staining assay showed that the death rate of cells in the combination group（53.58±1.57） % was significantly higher than the 5-Aza-2＇-dc group（7.44 ± 2.31） %and paraquat group（12.90 ±1.21） %（P〈0.05）. Conclusion 5-Aza-2＇-dc promotes V79 cells damage caused by paraquat.

关 键 词：5-Aza-2'-dc 百草枯 V79细胞 毒性 

分 类 号：R114[医药卫生—卫生毒理学]