3D打印纳米羟基磷灰石/聚己内酯支架生物相容性  

BIOCOMPATIBILITY OF THREE-DIMENSIONAL PRINTED NANO-HYDROXYAPTITE/POLYCAPROLACTONE SCAFFOLD

作　　者：余程[1] 刘凯[2] 侯长江[1] 何涛[1] 邓文帅[1] 孙鹏[1] YU Cheng LIU Kai HOU Changjiang HE Tao DENG Wenshuai SUN Peng(Department of Neurosurgery, The Affiliated Hospital of Qingdao University, Qingdao 266555, Chin)

机构地区：[1]青岛大学附属医院神经外科,山东青岛266555 [2]日照市人民医院

出　　处：《青岛大学医学院学报》2017年第1期1-4,共4页Acta Academiae Medicinae Qingdao Universitatis

基　　金：国家自然科学基金资助项目(81671305);十二五国家科技支撑计划项目(2013BAI01B03);山东省重点研发项目(2015-GSF118177);青岛市自主创新重大科技专项(14-6-1-6-zdzx)

摘　　要：目的探讨3D打印纳米羟基磷灰石/聚己内酯支架的生物相容性。方法分离培养SD大鼠骨髓间充质干细胞,将3D打印纳米羟基磷灰石/聚己内酯支架与骨髓间充质干细胞复合培养,用扫描电镜观察细胞在支架上黏附生长情况。采用CCK-8法检测3D打印纳米羟基磷灰石/聚己内酯支架浸提液(实验组)和DMEM完全培养液(对照组)对骨髓间充质干细胞的细胞毒性,倒置相差显微镜下观察两组细胞形态变化。采用溶血试验检测3D打印纳米羟基磷灰石/聚己内酯支架浸提液对新鲜抗凝兔血的溶血反应,以生理盐水为阴性对照,双蒸水为阳性对照。结果扫描电镜观察显示,3D打印纳米羟基磷灰石/聚己内酯支架呈多孔隙网状结构,骨髓间充质干细胞能在支架上黏附生长。倒置相差显微镜观察显示,骨髓间充质干细胞在支架浸提液中生长良好,实验组与对照组细胞形态无明显差别。CCK-8法检测显示,培养1、2、3、4d后实验组吸光度值与对照组比较差异均无显著性(P>0.05),受试支架的细胞毒性等级为1级。溶血试验结果显示,支架浸提液溶血率为1.16%。结论 3D打印纳米羟基磷灰石/聚己内酯支架具有良好的生物相容性,有望成为一种新型的颅骨缺损修复材料。Objective To investigate the biocompatibility of three-dimensional（3D）printed nano-hydroxyaptite/polycaprolactone（nHA/PCL）scaffold. Methods Bone marrow mesenchymal stem cells（BMSCs）from Sprague-Dawely rats were isolated and co-cultured with 3D printed nHA/PCL scaffold,the growth and adhesion of cells on the scaffold surface were observed using scanning electron microscope（SEM）.Applying CCK-8assay,the cytotoxicity of 3D printed nHA/PCL scaffolds extract（experimental group）,and DMEM medium（control group）on bone marrow mesenchymal stem cells was detected.Cell morphology of experimental group and control group was observed under an inverted phase contrast microscope.The extract of 3D printed nHA/PCL scaffolds on hemolytic reaction of fresh anticoagulant rabbit blood was detected-normal saline serving as negative control,and double distilled water as positive control-applying hemolysis test. Results The results of SEM showed that nHA/PCL scaffolds had a porous mesh structure,and BMSCs adhered and grew well on the surface of 3D printed nHA/PCL scaffolds.The results of inverted phase contrast microscope showed that cells grew well in 3D printed nHA/PCL scaffolds extract,and there was no difference in cell morphology between experimental group and control group.After 1,2,3 and 4days of culture,the results of CCK-8showed the difference in absorbance value between the experimental group and the control group was not significant（P〉0.05）,the cytotoxicity of scaffolds was grade 1.The results of hemolysis test showed the hemolysis ratio of 3D printed nHA/PCL scaffolds was 1.16%. Conclusion 3D printed nHA/PCL scaffold has good biocompatibility and it is expected to be a new material for repairing skull defect.

关 键 词：组织支架 3D打印 聚己内酯 羟基磷灰石类 生物相容性试验 

分 类 号：R318.08[医药卫生—生物医学工程] R651.1[医药卫生—基础医学]