青岛和广州地区人群鼻咽癌组织EBNA2基因多态性比较  

EPSTEIN-BARR VIRUS EBNA2 POLYMORPHIC PATTERNS IN NASOPHARYNGEAL CARCINOMA BETWEEN POPULATION IN QINGDAO AND GUANGZHOU

作　　者：张超[1,2] 吴国才[3] 晁艳[4] 刘颂[1] 舒君[1] 罗兵[1] 

机构地区：[1]青岛大学医学院微生物学教研室,山东青岛266021 [2]青岛市第九人民医院检验科 [3]青岛大学附属医院黄岛分院检验科 [4]广东省中医院检验科

出　　处：《齐鲁医学杂志》2017年第1期1-3,6,共4页Medical Journal of Qilu

基　　金：国家自然科学基金项目(NSFC81571995)

摘　　要：目的比较青岛和广州地区EB病毒(EBV)阳性鼻咽癌(NPC)组织中EBV核抗原2(EBNA2)基因多态性,并探讨EBV基因亚型与NPC发生的关联性。方法选择广州地区32例EBV阳性NPC组织及39例EBV阳性健康成人咽漱液(TW),采用PCR技术对其EBNA2基因编码aa331-487片段序列进行扩增和测序分析。结果 E2-A亚型为广州地区EBNA2的主要型别,NPC组织中EBNA2基因亚型的分布明显高于健康成人TW,差异有显著性(χ~2=12.2,P<0.01);E2-A亚型也为青岛地区主要亚型,但广州地区NPC组织中E2-A亚型检出率明显高于青岛地区(χ~2=18.4,P<0.01);B95-8样亚型为TW的优势亚型。结论 E2-A在EBV阳性NPC中高频出现提示该亚型可能与NPC的发生相关;B95-8样亚型为正常健康人群的优势亚型;EBNA2基因变异具有肿瘤相关性而非仅仅地域相关性。Objective To compare Epstein-Barr virus（EBV）nuclear antigen 2（EBNA2）encoding gene polymorphism in EBV positive nasopharyngeal carcinoma（NPC）tissue in crowd of Qingdao and Guangzhou and explore the correlation between EBV gene subtypes and NPC. Methods Tissues of EBV positive NPC were taken from 32 patients,and throat washing（TW）samples collected from 36 EBV positive healthy individuals of Guangzhou.Employing PCR technique,amplification and sequencing were done for EBNA2aa331-487 gene. Results The subtype E2-A was the main type of EBNA2 in Guangzhou.In NPC tissue,the distribution of EBNA2 subtypes was higher than that in TW of healthy adults,the difference was significant（χ^2=12.2,P〈0.01）,and E2-A subtype was also a main type found in Qingdao,but the detection rate of E2-A in NPC tissue was much higher in Guangzhou versus that in Qingdao（χ^2=18.4,P〈0.01）.B95-8-like subtype was the dominant type in TW. Conclusion High frequency of E2-A in Epstein-Barr virus positive nasopharyngeal carcinoma suggests that E2-A may be associated with the occurrence of the cancer.B95-8-like subtype is the dominant type in healthy people.EBNA2 gene mutation has a tumor correlation rather than a regional correlation.

关 键 词：疱疹病毒4型 人 鼻咽肿瘤 抗原 核 多态性 单核苷酸 

分 类 号：R373.9[医药卫生—病原生物学]