尿酸盐晶体对成骨前体细胞增殖及其分化的影响  

EFFECT OF MONOSODIUM URATES CRYSTALS ON THE PROLIFERATION AND DIFFERENTIATION OF 3T3-E1 CELLS

作　　者：王宾[1] 徐丽丽[1] 罗欢[1] 宋利娜[1] 郑芳芳[1] 杨乃龙[1] 

机构地区：[1]青岛大学附属医院内分泌科,山东青岛266003

出　　处：《齐鲁医学杂志》2017年第1期54-56,共3页Medical Journal of Qilu

摘　　要：目的观察尿酸盐晶体(MSU)对成骨前体细胞生长及分化的影响。方法取培养至对数期的成骨前体细胞3T3-E1,对照组不加入MSU,实验组分别加入0.02、0.06、0.10、0.20、0.50g/L的MSU刺激培养48h后,应用MTT法观察各组细胞生长情况;另取对数期的成骨前体细胞3T3-E1,分别加入成骨诱导剂,对照组不加入MSU,实验组分别加入浓度为0.02、0.10g/L的MSU,诱导14d后,行碱性磷酸酶染色,并提取RNA样品,采用RNA实时荧光定量PCR技术(RT-PCR)测定核心结合因子a1(Runx2)、锌指结构转录因子(Osterix)、骨钙素(Osteocalcin)与Ⅰ型胶原酶(Collal 1)的表达。结果 MSU能够抑制成骨前体细胞3T3-E1的增殖,且浓度越高抑制作用越明显(F=560.74,q=8.52~63.52,P<0.05);碱性磷酸酶染色显示,随着MSU浓度的增加染色变浅;RTPCR检测显示,Runx2、Osterix、Osteocalcin、Collal 1基因的表达量也随着MSU浓度的增加而降低(F=31.06~112.47,q=3.82~20.57,P<0.05)。结论 MSU能够抑制成骨前体细胞的增殖,而且能够抑制其向成骨细胞的分化及分泌。Objective To observe the effect of monosodium urates（MSU）on the proliferation and differentiation of 3T3-E1 cells. Methods 3T3-E1 cells were induced to differentiate into osteoblast with different concentrations of MSU-0,0.02,0.06,0.10,0.20,0.60g/L.After 48 hof stimulation culture,growth of the cells in each group was observed applying MTT method.3T3-E1 cells were taken and osteogenic induce supplement added.MSU was not added to the control group.MSU-0.02and0.10g/L-was added to the experimental group.After 14 hof induction,alkaline phosphatase staning was done and RNA samples were extracted.Applying RNA real time fluorecent quantitative PCR,the expressions of Runx2,Osterix,Osteocalcin,and Collal 1were detected. Results MSU could inhibit 3T3-E1 proliferation,it became strong with the increase of its concentration（F=560.74,q=8.52-63.52,P〈0.05）.The alkaline phosphatase staining revealed the color became lighter with the increase MSU concentration.RT-PCR indicated that the expressions of Runx2,Osterix,Osteocalcin and Collal 1declined with the increase of MSU concentration（F=31.06-112.47,q=3.82-20.57,P〈0.05）. Conclusion Monosodium urates can inhibit osteoblast precursor cell（3T3-E1）proliferation and its differentiation into osteoblast and secretion.

关 键 词：痛风 尿酸盐晶体 成骨细胞 细胞增殖 

分 类 号：R589.7[医药卫生—内分泌]