黄精多糖对去卵巢大鼠骨质疏松模型中OPG和RANKL蛋白表达的影响  

The effect of Polygonatum Sibiricum Polysaccharide on the expression of OPG and RANKL in the rat model of ovariectomy-induced osteoporosis

作　　者：严芳娜 曾高峰[1] 宗少晖[2] 彭小明[2] 吴平平[1] 张磊[1] 何基琛 韦诚明 施雄智 

机构地区：[1]广西医科大学公共卫生学院,南宁市530021 [2]广西医科大学第一附属医院脊柱骨病外科,南宁市530021

出　　处：《实用医学杂志》2017年第8期1243-1246,共4页The Journal of Practical Medicine

基　　金：广西自然科学基金资助项目(编号:2015GXNS-FAA139150)

摘　　要：目的:探讨黄精多糖对去卵巢大鼠骨微结构、Ca、P、OPG和RANKL蛋白表达的影响。方法:5月龄雌性大鼠30只,随机分5组:假手术(Sham)组,去势(OVX)组,黄精多糖高、中、低(H-,M-,L-PSP)组。Sham组、OVX组灌胃生理盐水,PSP组灌胃黄精多糖。8周后,观察大鼠胫骨显微结构,Ca、P、OPG和RANKL蛋白表达。结果:OVX组体重、Ca、P、RANKL、骨小梁Tb.Sp较Sham组升高,骨小梁BV/TV、Tb.Th、Tb.N和OPG降低;H-,M-PSP组体重、PSP组Ca和H-PSP组P、H-PSP组RANKL、PSP组Tb.Sp较OVX组降低,H-,M-PSP组OPG和PSP组BV/TV、Tb.Th、Tb.N升高;差异具有统计学意义(P<0.05)。结论:黄精多糖对骨质疏松防治主要通过改善骨微结构、降低骨转换、提高OPG蛋白和降低RANKL蛋白表达。Objective To investigate PSP on bone microstructures, Ca, P, OPG and RANKL of osteoporotic rat model. Methods Thirty female rats randomly divided into five groups：Sham, OVX, H-, M-, L-PSP. Sham and OVX were irrigated stomachsaline; PSP solution was gavaged to other groups. After 8-week, bone microstructures of tibial metaphyseal, Ca, P, OPG and RANKL were measured. Results Body weight, Ca, P, RANKL, Tb.Sp of OVX were significantly increased compared to Sham, OPG, BV/TV, Tb.Th, Tb.N decreased. Body weight of H-, M-PSP, Ca and Tb.Sp of PSP, P and RANKL in H-PSP were decreased compared to OVX, OPG in H-, M-PSP, BV/TV, Tb. Th, Tb.N of PSP group increased. The differences were statistically significant （P 〈 0.05）. Conclusion PSP pre- vents osteoporosis by improving the microstructure of trabecular bone, reducing bone turnover, increasing OPG and reducing RANKL expression.

关 键 词：黄精多糖 骨质疏松 OPG RANKL 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]