Caveolin-1在甘精胰岛素改善2型糖尿病小鼠糖代谢及骨骼肌炎症中的作用  

Role of Caveolin-1 in glargine in anti-inflammation and glycemic metabolism improvement of type 2 diabetic mice

作　　者：彭航娅 李海成[1] 林硕[1] 曾文[1] 林楚文[1] 林可意[1] 曾龙驿[1] 

机构地区：[1]中山大学附属第三医院内分泌与代谢病,广东省糖尿病重点实验室,广州市510075

出　　处：《实用医学杂志》2017年第8期1247-1249,共3页The Journal of Practical Medicine

基　　金：国家自然基金(编号:81070661);诺和诺德中国糖尿病英才研究基金(2014)

摘　　要：目的:观察Caveolin-1下调的2型糖尿病小鼠接受甘精胰岛素治疗后糖代谢及骨骼肌炎症的改善情况。方法:C57BL/6J小鼠随机分为2组:对照组(NC组)和2型糖尿病模型组(T组)。T组采用高脂高糖饮食加小剂量链脲佐菌素腹腔注射方法成模。T组根据胰岛素治疗及尾静脉接受病毒注射情况再分为4组:无干预组(T2DM组),接受胰岛素治疗组(insulin组),注射sh RNA沉默Caveolin-1表达并胰岛素治疗组(LV-sh RNACav1组),注射阴性病毒并胰岛素治疗组(LV-GFP),治疗组均皮下注射甘精胰岛素3周,记录小鼠随机血糖及胰岛素剂量,取小鼠骨骼肌用Western blot检测Caveolin-1及TNF-α的表达。结果:与insulin组及LV-GFP组小鼠相比,LV-sh RNACav1组小鼠控制血糖需要的甘精胰岛素量最大(P<0.05),骨骼肌中炎症因子TNF-α表达量最多(P<0.05)。结论:Caveolin-1与机体胰岛素敏感性相关,并可能参与了胰岛素对骨骼肌炎症的改善作用。Objective To investigate the role of glargine in glucose metabolism improvement and anti- inflammation of skeletal muscle in Caveolin-1 silenced type 2 diabetic mice. Methods Muhiple low doses of streptozotocin （STZ） intraperitoneal injection and high-fat high-glucose （HFHG） were used to induce type 2 diabetic mice model. The mice were divided into normal control group （NC group） and type 2 diabetic model group （T group）. Then according to virus injection and glargine treatment, T group were further divided into type 2 diabetes group （T2DM group）, type 2 diabetes with insulin treatment group （insulin group）, Caveolin-1 silenced with insulin treatment group （LV-CAV1 group） , and scramble virus with insulin treatment group （LV-GFP group）. Glucose metabolism was accessed by the fluctuation of blood glucose. TNF-α protein expression in skeletal muscle was detected by Western blot. Results The glycemic control of LV-CAV1 group needed more dosages of glargine （P 〈 0.05）. The expression of TNFαin skeletal muscle was elevated in LV-CAV1 group than that in LV- GFP and insulin group （P 〈 0.05）. Conclusion The antiinflammation function and glycemic metabolism improvement of glargine may be associated with the expression of Caveoin- 1 in skeletal muscle.

关 键 词：CAVEOLIN-1 骨骼肌 2型糖尿病 TNF-Α 甘精胰岛素 

分 类 号：R587.1[医药卫生—内分泌]