雌二醇缩短哮喘小鼠引喘潜伏期并促进气道炎症  

Estrogen shortens the latent period of inducing asthma and promotes airway inflammation

作　　者：冯帅 朱述阳 韩方方 朱洁晨 张毛为 刘文静 

机构地区：[1]徐州医科大学附属医院呼吸内科,江苏徐州221006

出　　处：《细胞与分子免疫学杂志》2017年第4期450-454,共5页Chinese Journal of Cellular and Molecular Immunology

摘　　要：目的观察雌二醇对哮喘小鼠引喘潜伏期及气道炎症的影响。方法雄性BALB/c小鼠随机分为正常对照组、卵清蛋白(OVA)诱发哮喘组、400μg/kg雌二醇处理组、400μg/kg雌二醇联合7 mg/kg他莫昔芬组,每组10只。OVA诱发哮喘组、雌二醇处理组、雌二醇联合他莫昔芬组通过OVA致敏、激发建立哮喘模型,正常对照组以生理盐水替代。雌二醇处理组、雌二醇联合他莫昔芬组于每次激发前4 h给予400μg/kg雌二醇腹腔注射,雌二醇联合他莫昔芬组在注射雌二醇前30 min给予7 mg/kg他莫昔芬腹腔注射。正常对照组、OVA诱发哮喘组腹腔注射生理盐水作对照。于末次激发后24 h,测定各组小鼠引喘潜伏期。收集支气管肺泡灌洗液(BALF)检测白细胞总数并进行嗜酸性粒细胞和淋巴细胞分类计数,ELISA检测BALF中白细胞介素4(IL-4)、IL-13水平。HE染色观察肺组织病理变化。结果与正常对照组比较,OVA诱发哮喘组、雌二醇处理组、雌二醇联合他莫昔芬组引喘潜伏期均缩短;雌二醇处理组较OVA诱发哮喘组、雌二醇联合他莫昔芬组引喘潜伏期缩短;OVA诱发哮喘组与雌二醇联合他莫昔芬组引喘潜伏期无显著性差异。与正常对照组比较,OVA诱发哮喘组、雌二醇处理组、雌二醇联合他莫昔芬组IL-4、IL-13水平升高;雌二醇处理组较OVA诱发哮喘组、雌二醇联合他莫昔芬组IL-4、IL-13水平升高;OVA诱发哮喘组与雌二醇联合他莫昔芬组IL-4、IL-13水平相当。与正常对照组比较,OVA诱发哮喘组、雌二醇处理组、雌二醇联合他莫昔芬组BALF白细胞总数及嗜酸性粒细胞、淋巴细胞分类计数增加;雌二醇处理组较OVA诱发哮喘组、雌二醇联合他莫昔芬组白细胞总数及嗜酸性粒细胞、淋巴细胞分类计数增加;OVA诱发哮喘组与雌二醇联合他莫昔芬组比较,白细胞总数及嗜酸性粒细胞、淋巴细胞分类计数无显著性差异。正常对照组小鼠气道黏�Objective To investigate the effect of estrogen on the latent period of inducing asthma and airway inflammation in mice with asthma. Methods Forty male BALB/c mice were randomiy divided into normal control group, OVA-induced asthma group, 400 μg/kg estradiol treatment group, 400 μg/kg estradiol combined with 7 mg/kg tamoxifen group, with 10 mice in each group. The OVA-induced asthma group, estradiol treatment group, and estradiol combined with tamoxifen group were sensitized and challenged by OVA; the normal control group was treated with normal saline instead. The estradiol treatment group and estradiol combined with tamoxifen group were given intraperitoneal injection of 400 μg/kg estradiol 4 hours before each challenge, and the latter was given additionally intraperitoneai injection of 7 mg/kg tamoxifen at 30 minutes before the injection of estradiol; the normal control group and OVA-induced asthma group were injected with normal saline as controls. The latent period of inducing asthma of all the mice was determined 24 hours after the final OVA challenge. Bronchoalveolar lavage fluid （BALF） was collected for counting total leukocyte cells and the percentages of eosinophils and lymphocytes. The concentrations of interleukin 4 （IL-4） and IL-13 in BALF were detected by ELISA. The pathologic change of lung tissues was examined by HE staining. Results Compared with the normal control group, the latent period of inducing asthma were shortened in the OVA-induced asthma group, estradiol treatment group, and estradiol combined with tamoxifen group; compared with the OVA-induced asthma group and estradiol combined with tamoxifen group, the latent period of inducing asthma of the estradiol treatment group were shortened; there was no significant difference in the latent period between the OVA-induced asthma group and the estradiol combined with tamoxifen group. Compared with the normal control group, the levels of IL-4 and IL-13 increased in the OVA-induced asthma group, estradiol treatment group, and estradio

关 键 词：雌二醇 哮喘 引喘潜伏期 气道炎症 

分 类 号：R256.12[医药卫生—中医内科学] R392.12[医药卫生—中医学]