HPLC法测定四环素可的松眼膏中盐酸四环素的含量  

HPLC DETERMINATION OF TETRACYCLINE HYDROCHLORIDE IN TETRACYCLINE AND CORTISONE EYE OINTMENT

作　　者：陈伟[1] 仇士林[1] 刘谷欲[1] 

机构地区：[1]上海市药品检验所,上海200233

出　　处：《中国药品标准》2002年第4期50-52,共3页Drug Standards of China

摘　　要：目的:建立HPLC法测定四环素可的松眼膏中盐酸四环素的含量。方法:盐酸四环素经0.01mol/L盐酸溶液提取后,采用Komasil C_(18)柱,0.1mol/L草酸铵-N,N-二甲基甲酰胺0.2mol/L磷酸氢二铵(68:27:5)(用氨试液调pH值为8.3)为流动相,280nm为检测波长。结果:盐酸四环素平均回收率为100.1％,RSD=0.9％(n=8)。结论:本方法简便,快速,结果准确。Objective:To eatablish a method for determination of tetracycline hydrochloride content inTetracycline Cortisone Eye Ointment. Method: Tetracycline hydrochloride was extracted with 0.01mol/Lhydrochloric acid and determined by HPLC. Using Komasil C_(18) as column 0.1mol/L ammonium oxalate-N,N-dimethylformamide-0.2mol/L diammonium hydrogen phosphate(68:27:5) (adjused the solution withammonium TS to pH8.3)as the mobile phase and detecting at 280nm. Results: The average recovery was100.1%, RSD was 0.9% (n=8). Conclusion: The method is simple,quick and accurate.

关 键 词：四环素可的松眼膏 盐酸四环素 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]