抵抗素、miRNA-26b在婴幼儿毛细支气管炎中的表达  

Expressions of resistin and miRNA-26b in bronchiolitis in infants

作　　者：许长娣[1] 周瑶[1] 张佳敏[1] 赵德育[1] 刘峰[1] 

机构地区：[1]南京医科大学附属儿童医院呼吸科,南京210008

出　　处：《安徽医科大学学报》2017年第5期752-755,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81200012)

摘　　要：目的探讨婴幼儿毛细支气管炎中miRNA-26b、抵抗素的变化。方法收集毛细支气管炎患儿的血液,以正常儿童为对照,通过酶联免疫吸附(ELISA)法测定血清中白介素(IL)-8、抵抗素浓度,并利用实时定量PCR技术测定血液淋巴细胞中miRNA-26b、抵抗素mRNA表达,利用miRNA-26b mimics干预人肺上皮细胞A549,通过实时定量PCR技术测定miRNA-26b、抵抗素mRNA及细胞上清液中IL-8蛋白浓度变化。结果毛细支气管炎患儿外周血清中IL-8、抵抗素浓度均升高,血液淋巴细胞中miRNA-26b、抵抗素mRNA表达增加;经过干预的A549细胞miRNA-26b、抵抗素mRNA及细胞上清液中IL-8蛋白浓度均有增加。结论 miRNA-26b、抵抗素在毛细支气管炎患儿血液淋巴细胞中存在差异表达,而这可能参与了炎症反应的发生。Objective To study the expression and slinical significance of miRNA-26b, resistin and inflammatory mediator in bronchiolitis. Methods Serum IL-8, resistin concentration （ by enzyme linked immunosorbent assay, ELISA）, microRNA（miRNA）-26b with resistin mRNA expression in blood lymphocytes（ by real-time quantitative PCR assay） were analyzed in children with bronchiolitis and healthy age-match control. After miRNA-26b mimics treating human lung epithelial cells A549, IL-8 concentrations in cell supernatants, miRNA-26b and resistin in cells were analyzed. Results In children with bronchiolitis, serum IL-8, resistin protein concentrations were inc- reaced, as well as miRNA-26b and resistin mRNA in blood lymphocytes. After the intervention of A549 cells miR- NA-26b, resistin mRNA and IL-8 protein concentrations in supernatants were increased. Conclusion miRNA-26b and resistin expression change in blood lymphocytes in children with bronchiolitis, which may be involved in the in- flammatory response.

关 键 词：抵抗素 MICRORNA 人肺上皮细胞 IL-8 呼吸道合胞病毒 

分 类 号：R562.21[医药卫生—呼吸系统]