荒漠昆虫小胸鳖甲转录组微卫星的生物信息学分析  

Bioinformatic Analysis of the Microsatellites from the Transcriptome of Desert Beetle Microdera punctipennis

作　　者：库尔班·吐松[1] 陈真[1] 刘小宁[1] 马纪[1] 

机构地区：[1]新疆大学生命科学与技术学院,新疆生物资源基因工程重点实验室,乌鲁木齐830046

出　　处：《基因组学与应用生物学》2017年第3期865-873,共9页Genomics and Applied Biology

基　　金：国家自然科学基金项目(31360527)资助

摘　　要：位于蛋白质编码区及非编码区(可转录区)的微卫星是常用的一种功能分子标记。本研究以荒漠拟步甲科昆虫小胸鳖甲(Microdera punctipennis)为材料,从其转录组数据库中筛选出由1~6个碱基单元组成的简单序列重复进行分析,并对其微卫星的丰度和特征进行描述。研究显示,小胸鳖甲转录组中微卫星的分布频率为7.94%。其中,单碱基重复序列占微卫星的47.17%,是最丰富的重复序列;其次为3碱基重复序列,占39.81%,而2、4、5、6碱基重复序列分别占10.94%、0.90%、0.88%和0.29%。将小胸鳖甲转录本与同为拟步甲科的赤拟谷盗(Tribolium castaneum)编码序列进行比对,分析同源序列微卫星基元(motif)发现,二者蛋白质编码区微卫星具有高度物种特异性。对小胸鳖甲低温响应转录本和总转录本进行微卫星基元和不同长度微卫星重复次数的比较发现,它们之间没有显著性差异。对含有微卫星的冷响应基因进行基因本体论(gene ontology,GO)分析,发现主要富集到生物学过程、分子功能和细胞组分3个类群。Microsatellites were located in coding regions and untranslated （transcribable） regions are commonly used as functional molecular markers. In this study, the transcriptome data of the desert beetle Microdera punctipennisin （Tenebrionidae） was screened for simple sequences from 1 to 6 nucleotide repeating units, and analyzed their abundance and characteristics. The results revealed that the distribution frequency of the microsatellites in the transcriptome was 7.94%, of which the single nucleotide repeats were the most abundant repeat type, and accounted for 47.17% of the total microsatellites, closely followed by tri-nucleotide repeats （39.81%）. The di-, tetra-, penta- and hexa-nucleotide repeats accounted for 10.94%, 0.90%, 0.88% and 0.29% respectively. The microsatellite motifs of the homologous sequences between the transcripts of M. punctipennis and the red flour beetle Tribolium castaneum which also belongs to Tenebrionidae were compared, the results showed that microsatellites in protein coding regions are highly species specific between the two insects. When compared the microsatellite motifs and the repetition times of microsatellites with different lengths between the cold responsive transcripts and the total transcripts of M. punctipennis transcriptome, we found no significant differences between them. Gene ontology （GO） analysis of the microsatellites in cold responsive transcripts enriched them into three terms： biological processes, molecular functions and cellular components.

关 键 词：小胸鳖甲 转录组 微卫星 特异性 冷响应 

分 类 号：Q963[生物学—昆虫学]