丹酚酸B对TGF-β1诱导大鼠粘连成纤维细胞增殖和细胞外基质合成的影响  

Effects of salvianolic-acid B on proliferation and extracellular matrix synthesis in rat adhesive fibroblasts induced by transforming growth factor-beta1

作　　者：卢彬[1] 王芳[1] 蒋泓[2] 曾煦欣[2] 龙晓英[1] 

机构地区：[1]广东药科大学中药学院,广州510006 [2]佛山科学技术学院口腔医学院,佛山528000

出　　处：《中国新药杂志》2017年第8期930-935,共6页Chinese Journal of New Drugs

基　　金：广东省自然科学基金资助项目(2015A030313634)

摘　　要：目的:研究丹酚酸B(SAB)对转化生长因子-β1(TGF-β1)诱导大鼠粘连组织成纤维细胞(ATF)增殖活性和胞外基质(ECM)合成的影响。方法:采用Cell Count Kit法(CCK-8)检测SAB对TGF-β1诱导ATF增殖影响。实时荧光定量PCR技术(Real-time PCR)、酶联免疫吸附法(ELISA)检测SAB对TGF-β1诱导ATF I型胶原蛋白(COLI)、纤维连接蛋白(FN)、金属基质蛋白酶-9(MMP-9)和金属基质蛋白酶抑制剂-1(TIMP-1)mRNA和蛋白表达的影响。结果:10 ng·m L^(-1)TGF-β1可显著提高细胞外基质成分COLI和FN的表达。10μmol·L^(-1)SAB可以抑制TGF-β1诱导ATF增殖活性(P<0.05),并显著下调COLI和FN的表达;另外还可促进MMP-9表达、降低TIMP-1活性,调节细胞外基质降解平衡。结论:SAB预防术后腹膜粘连的机制与抑制TGF-β1诱导的ATF增殖,调节MMP-9和TIMP-1分泌平衡促进ECM降解有关。Objective： To study the effects of salvianolic-acid B（ SAB） on proliferation and extracellular matrix（ ECM） synthesis in rat adhesive tissue fibroblasts（ ATF） induced by transforming growth factor beta-1（ TGF-β1）. Methods： The proliferation of ATF was detected by Cell Count Kit（ CCK-8）. The gene expressions of collagen I（ COLI）,fibronectin（ FN）,matrix metalloproteinase-9（ MMP-9） and tissue inhibitor of metalloproteinase-1（ TIMP-1） were detected by real-time PCR. ELISA was employed to determine the protein levels of COLI,FN,MMP-9 and TIMP-1. Results： TGF-β1 of 10 ng·m L-1could effectively up-regulate the COLI and FN expressions.SAB of 10 μmol·L-1could suppress the ATF proliferation（ P 〈 0. 05） and down-regulate the expressions of COLI and FN in TGF-β1-stimulated ATF. Besides,SAB could also significantly up-regulate MMP-9 while down-regulate TIMP-1 in TGF-β1-stimulated ATF. Conclusion： SAB can inhibit TGF-β1-induced ATF proliferation. It can also restore the balance of MMP-9 and TIMP-1 expression in ATF,thus improve the ECM degradation,which may be the preventive and prophylactic mechanisms of SAB on post-operative adhesion.

关 键 词：丹酚酸B 转化生长因子-Β1 粘连成纤维细胞 细胞外基质 

分 类 号：R285.5[医药卫生—中药学]