红球菌R04细胞分裂相关蛋白RHOGL008438对大肠杆菌细胞丝状化的影响  

Effect of Rhodococcus sp. R04 Cell-division-related Protein RHOGL008438 on the Filaments Formation of Escherichia coli

作　　者：许晓潇 杨秀清[1] 

机构地区：[1]山西大学生物技术研究所,化学生物学与分子工程教育部重点实验室,山西太原030006

出　　处：《山西大学学报（自然科学版）》2017年第2期373-379,共7页Journal of Shanxi University(Natural Science Edition)

基　　金：山西省自然科学基金(2014011030-3);山西省煤层气联合研究基金(2015012002);山西省煤基重点科技攻关项目(MQ2014-03)

摘　　要：通过转录组测序分析发现红球菌R04的RHOGL008438蛋白可能与细胞分裂有关。为研究其生理功能,通过构建融合表达质粒在大肠杆菌BL21中异源表达RHOGL008438蛋白,并在荧光显微镜下观察该蛋白对大肠杆菌细胞形态的影响。结果表明,过量表达RHOGL008438蛋白会使大肠杆菌细胞出现丝状化,丝状化程度与IPTG诱导浓度呈剂量效应关系,且培养时间越长,细胞丝状化程度越高。IPTG浓度为0.5mmol/L时培养5h后细胞长度可达46.2μm,表明RHOGL008438蛋白干扰了大肠杆菌细胞隔膜的正常形成导致细胞丝状化。通过序列同源性分析,确定RHOGL008438蛋白为细胞分裂过程中的主要分裂蛋白FtsZ。Transcriptomics analysis indicated that the protein RHOGL008438 of Rhodococcus sp. R04 may be related to cell division. The heterologous expression of RHOGL008438 in Escherichia coli BL21 was performed to find out its physiological function on cellular morphology by fluorescence microscope. The results showed that the filamentous cells were formed. There was a significant correlation between cell filamentous and IPTG concentration. The longer the culture time was, the longer the filamentous was. The cell length of E. coli was reached 46.2 μm after 5 h under 0.5 mmol/L IPTG. The results revealed that RHOGL008438 inhibited the formation of septum of E. coli, and confirmed it played a significant role in cell division. RHOGL008438 was then identified as cell-division-related protein FtsZ by sequence homology analysis.

关 键 词：红球菌R04 细胞分裂蛋白 细菌细胞分裂 异源表达 

分 类 号：Q93[生物学—微生物学] Q25