高通量测序及其在食物网解析中的应用进展  

Progress on high-throughput sequencing and its applications in food web analysis

作　　者：王雪芹[1,2] 王光华[1,2] 乔飞[1,2] 高其康[2,3] Kong Luen HEONG 祝增荣[1,2] 程家安[1,2] 

机构地区：[1]浙江大学水稻生物学国家重点实验室,杭州310058 [2]浙江大学昆虫科学研究所,杭州310058 [3]浙江大学农生环学部分析测试中心,杭州310058

出　　处：《生态学报》2017年第8期2530-2539,共10页Acta Ecologica Sinica

基　　金：国家科技支撑计划项目(2012BAD19B01);浙江省自然科学基金项目(LY16C140002);科技部国家"973"基础重点研究发展规划项目(2010CB126200)

摘　　要：高通量测序是DNA测序技术发展中的重大突破,它的出现为现代生物科学研究提供了前所未有的机遇,例如基于猎物和寄主植物DNA分子解析生态系统的食物网研究已逐渐成为捕食性动物与植食性动物食物网研究的新型模式。在简要总结Roche 454、Illumina和Ion Torrent为代表的第二代测序技术的原理及最新进展的基础上,综述了近年来利用高通量测序技术在捕食性和植食性动物食物网解析构建研究方面取得的最新进展及存在的问题,以期为探索捕食性和植食性动物的猎物/寄主范围、猎物/寄主转换、资源分配、生物防治、生物保护和生态恢复新方法提供思路和启发。High-throughput sequencing is a major breakthrough in the development of DNA sequencing technology andprovides an unprecedented opportunity for modern biological scientific research, such as the DNA-based approach trackingthe food chains among predators-prey or herbivores-host plants trophic interactions in ecosystems. This review illustrates andcompares the principles of various types of technology, including the Roche 454, Illumina, and Ion Torrent technologies,and other recent progress. We also summarize studies that have used high-throughput sequencing technology to studyinteractions among generalist predators/herbivores and their prey/hosts. This review could provide new information andnovel approaches to exploring molecular trophic interactions and improving our understanding of the prey/host spectrum,prey/host shift, biological control, resource allocation, conservation biology, and ecological restoration.

关 键 词：第二代测序 捕食/植食性动物 营养关系 食物转换 宏条形码技术 

分 类 号：Q148[生物学—生态学]