气相色谱法检测活菌型乳酸菌饮料中乙醇质量浓度  

Determination of ethanol in the milk beverage with live lactobacillus by Gas Chromatography

作　　者：韩磊 李光辉 吴小慧 金珠 

机构地区：[1]蒙牛乳业(马鞍山)有限公司,安徽马鞍山243000

出　　处：《中国乳品工业》2017年第4期59-61,共3页China Dairy Industry

摘　　要：建立了活菌型乳酸菌饮料中乙醇质量浓度的气相色谱检测方法。采用乙腈沉淀样品中蛋白质,离心后取上清液用强极性的CD-2560(100 m×0.25 mm×0.2μm)毛细管色谱柱分离,氢火焰离子化检测器检测,并以保留时间定性和峰面积定量。该方法简便、快速、实用、准确,可用于活菌型乳饮料中乙醇质量浓度的快速检测。Gas chromatography is established to determine the athanol contents of beverage with live lactobaeillus.Take acetonitrile to precipi- tate sample protein,take supernatant hquid after centrifugation,separate by capillary gas chromatographic columns with CD-2560 （100 m× 0.25 mm×0.2 μm）, detect by hydrogen flame ionization detector,and have retention time and peak height or peak area.This method is sim- ple, fast, practical and accurate, and can be quickly used to detect ethanol content in the milk beverage with live bacteria.

关 键 词：气相色谱 活菌型乳酸菌饮料 乙醇 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]