氟铝联合持续暴露对二代仔鼠神经生理发育及海马结构的影响  

Effects of the continuous fluorine combined with aluminum exposure on neurobehavior physiogenesis and hippocampus structures of the 2^(nd) offspring

作　　者：吴宇[1,2] 张华[1] 谢春[1] 杨小雪[3] 陈媛[1] 葛启迪 

机构地区：[1]贵州医科大学公共卫生学院环境卫生学教研室,贵州贵阳550001 [2]毕节医学高等专科学校,贵州毕节551700 [3]贵州省黔东南州疾病预防控制中心,贵州黔东南州556099

出　　处：《现代预防医学》2017年第9期1668-1671,1675,共5页Modern Preventive Medicine

基　　金：国家自然科学基金地区基金资助(81560519)

摘　　要：目的探讨氟铝联合持续暴露对二代仔鼠神经生理发育和海马结构的影响。方法 SD孕鼠36只,随机分为9组(每组4只):对照组和单独氟化钠(100、200 mg/L)、三氯化铝(500、1 000 mg/L)染毒组及氟化钠+三氯化铝联合染毒组。从母鼠妊娠第0天至二代仔鼠出生第60天(PND60),自由饮水染毒。每组一代仔鼠于PND90(性成熟)随机选取6只(雌雄比为2∶1)合笼。每组二代仔鼠出生后,随机选取8只(雌雄各半),观察神经生理发育指标;PND60,收集12 h尿液,处死后留取大脑,离子选择电极法、电感耦合等离子体发射光谱法分别测定尿和脑中氟、铝;HE染色观察海马变化。结果与对照组比较,各染毒组脑重系数、开眼和出牙未见差异;高氟组、低氟低铝组和高氟低铝组平面翻正反射时间延长,高剂量氟、铝及氟铝联合组二代仔鼠悬崖回避反射时间延长(P<0.05)。HE染色显示高剂量氟、铝及氟铝联合组CA3区出现病理改变。结论高剂量氟、铝及氟铝联合可能致二代仔鼠的神经生理发育及海马损伤。Objective To explore the effects of the continuous fluorine combined with aluminum exposure on neurobehavior physiogenesis and hippocampus structures of the 2nd offspring. Methods Thirty - six pregnant rats were randomly divided into 9 groups（ four pregnant rats in each group）as follows：control group ,fluorine group（ drinking the water containing 100 mg/L and 200 mg/L sodium fluoride） ,aluminum group（ drinking the water containing 500 mg/L and 1 000 mg/L aluminum chloride） , fluorine plus aluminum group. The pregnant rats were treated with fluorine or aluminum through drinking water ad libitum from pregnant day 0 to postnatal day 21 of the first generation rats （PND21）. 6 offspring rats were selected randomly in each group and mated according to 2：1 when the first generation rats were at PND90.8 the 2nd offspring were selected randomly in each group and weighed interval 1 week. The pups were given the same treatment as their parental generation. Urine samples of 12 hours and brains of rats were collected. The concentrations of fluorine and aluminum in urine and brain were measured by ion selective electrode method and inductively coupled plasma atomic emission spectrometry, respectively. The hippocampus structure changes were observed after hematoxylin eosin（HE） staining. Results Compared with the control group, there was no significant difference in brain weight coefficients as well as teething and eye opening time from every test group. Time of surface righting reflex was significantly prolonged （ P 〈 0.05 ） in the high fluorine group, the low fluorine with low aluminum group as well as high fluorine with the low aluminum group. Time of cliff avoidance reflex was significantly prolonged （ P 〈 0.05 ） in the high dose fluorine as well as aluminum and fluorine combined with aluminum groups. HE staining results showed that CA3 area had detectable pathological changes in the high dose fluorine, aluminum and fluorine combined with the aluminum group. Conclusion The high - dose fluo

关 键 词：氟 铝 生理发育 神经行为 

分 类 号：R113[医药卫生—公共卫生与预防医学]